Alignments for a candidate for betS in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for betS in Pseudomonas fluorescens GW456-L13

Align Glycine betaine/proline betaine transporter BetS (characterized)
to candidate PfGW456L13_1085 High-affinity choline uptake protein BetT

Query= SwissProt::G3XCN6
         (706 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1085
          Length = 665

 Score =  469 bits (1208), Expect = e-136
 Identities = 253/649 (38%), Positives = 374/649 (57%), Gaps = 14/649 (2%)

Query: 49  QRFKVNLPVFVGSVAVIALFVGIGVIAPKRAESIFSGMQTAILSGFGWLYLLSVAVFLFS 108
           ++  VN  VF  S A+I L   I +IAP+ A  +    Q  +   FGW Y++ +A +L  
Sbjct: 12  EKVTVNGWVFYTSTALILLLTAILIIAPQEAGRMLGTAQAWLSRSFGWYYMVVIAAYLIF 71

Query: 109 MLFLAFSRYGELKLGPDDSEPEFRYLSWIAMLFAAGMGIGLMYFAVGEPMTHFASPPEAE 168
           ++ LAFS YG+LKLG  D  P+F Y +W  MLF++G+GI L+YF   EP+ H+ +PPE  
Sbjct: 72  VVGLAFSSYGKLKLGSKDDTPDFSYGAWAGMLFSSGIGISLLYFGASEPLDHYFNPPEGV 131

Query: 169 PLTIAAQREAMSVTFFHWGVHAWAIYSVVGLSLAYFGYRYNLPLTVRSGLYPLLKE-GIH 227
             +  A R+A+ +TF HWG+H WAIY++VGL++AYF YR+N PL +RS LYPL+ E  + 
Sbjct: 132 AGSNLAARQAVQLTFLHWGLHGWAIYALVGLAVAYFAYRHNQPLALRSALYPLVGERWVK 191

Query: 228 GPIGHVVDIFAICGTMFGLATSLGFGILQINSGLNYLLGIPQSIYVQLLLVTVVTAIATI 287
           G  GH VD F +  T+ GL T+LG G LQ++SGL  L G+  S    L+++ V++ +ATI
Sbjct: 192 GAAGHAVDGFGMFVTLLGLVTNLGIGSLQVSSGLENLFGMEHSNTNLLIVIIVMSTVATI 251

Query: 288 SVVTGVEKGVRILSETNLFLAVLLMLFVLVVGPTGTLMRDFVQNIGLYLDSLVLRTFNIY 347
           + V+GVE G+R LS  N+ L   L++FVL+ GPT  L+  FVQN+G YL+ +VL+TF++Y
Sbjct: 252 AAVSGVENGIRRLSNLNIVLFSGLLIFVLLFGPTLHLLNGFVQNVGDYLNGVVLKTFDLY 311

Query: 348 AYE-----PRPWIDSWTLFYWAWWISWSPFVGMFIARISRGRTVREFVTAVLFVPAMFTF 402
            YE        W+  WTLFYWAWWISW+PFVGMFIARISRGRTVRE V  VL +P  FT 
Sbjct: 312 VYEGDSEKSDRWLGLWTLFYWAWWISWAPFVGMFIARISRGRTVRELVAGVLLIPLGFTL 371

Query: 403 LWMTVFGNTAIYVDTTIANGELARDVKADLSVALFQFFEYLPWPAVTSTLAVLLVSIFFV 462
            W+++FGN+A+ +       EL +      S+A++Q  E+ P   V   +++ +  + F+
Sbjct: 372 AWLSIFGNSALDLVMNHGAVELGKTALEQPSMAIYQLLEHYPASKVVIGVSIFVGFVLFL 431

Query: 463 TSSDSGSLVIDTIASGG---ETATPALQRIFWCSLSGIVAAVLLSTGGLTALQSATISTA 519
           T +DSG++++  ++  G   +   P   RIFW ++  +V   LL  G   A+Q+  +   
Sbjct: 432 TPADSGAVMMANLSCKGGNVDEDAPHWLRIFWSAVITLVTIGLLFAGNFEAMQTMVVLAG 491

Query: 520 LPFSLVMLILVWSLFVGMRADLARTQSPGSLGPRAYPASGVPWQRRLAMTLSTPDRRAVE 579
           LPFS+V++  ++ L   MR D+   Q    L  R        +  RL      P +  V+
Sbjct: 492 LPFSVVLVFFMFGLHKAMRQDVQIEQEQAELAARGRRG----FSERLTQLDLQPSQSVVQ 547

Query: 580 KFLQASVLPALEAVARELTRRSRPASVGRDAETGALTLTVPAEGHRDFVYGVQMSEH-KL 638
           +F+   V PAL+  A +L  +              + + V  E    FVY V +  +   
Sbjct: 548 RFMDKHVTPALDDAAVQLRTQGLEVQTLLGKSKRCMGVRVEMEEGNPFVYEVSLDGYLAT 607

Query: 639 PAFTAYDATVADVRYEARTFFSDGSRGYDIMGMADNQIINDVLFQFERY 687
           P  +A         Y A  +  +GS+ YD+MG   +QI  DVL QFE +
Sbjct: 608 PTESAQSDEARQRYYRAEVYLHNGSQDYDLMGFTQDQITRDVLDQFESH 656


Lambda     K      H
   0.326    0.139    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1183
Number of extensions: 67
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 706
Length of database: 665
Length adjustment: 39
Effective length of query: 667
Effective length of database: 626
Effective search space:   417542
Effective search space used:   417542
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources