Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate PfGW456L13_1517 Acetyl-coenzyme A synthetase (EC 6.2.1.1)
Query= SwissProt::P27550 (652 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1517 Length = 645 Score = 793 bits (2048), Expect = 0.0 Identities = 374/629 (59%), Positives = 481/629 (76%), Gaps = 7/629 (1%) Query: 19 INPQQYEAMYQQSINVPDTFWGEQG-KILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNL 77 ++ Y+ +Y+QS+ P TFW EQ + LDW P++ V+ + G S W+ LN+ Sbjct: 19 LSQDDYQRLYRQSVEHPSTFWAEQATRFLDWSAPWETVQRYNLKTGEAS--WFAGAQLNV 76 Query: 78 AANCLDRHLQENGDRTAIIWEGDDASQSKHISYKELHRDVCRFANTLLELGIKKGDVVAI 137 + NC+DRHL++ GD+ AIIWEGDD + S I+YK+LH +VCR AN L G+KKGD V I Sbjct: 77 SYNCIDRHLEKRGDQVAIIWEGDDPADSTSITYKKLHENVCRLANVLKNRGVKKGDRVCI 136 Query: 138 YMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIP 197 YMPM+PEAA AMLAC RIGAVHSV+FGGFSP+++ RI+D++ R VIT+DEGVR G+ + Sbjct: 137 YMPMIPEAAYAMLACTRIGAVHSVVFGGFSPDSLRDRILDADCRTVITADEGVRGGKVVS 196 Query: 198 LKKNVDDALKN-PNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAE 256 LK+NVD AL++ P+V+SV +V++RT G ++W EGRD+ + + +AS E M+AE Sbjct: 197 LKQNVDKALQSCPDVSSV---LVVERTKGLVNWVEGRDIKYQQALREASADCPPEPMDAE 253 Query: 257 DPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYL 316 DPLFILYTSGSTGKPKGVLHTTGGYL+ AA+TFKYV DY +++WCTADVGWVTGHSY+ Sbjct: 254 DPLFILYTSGSTGKPKGVLHTTGGYLLQAAMTFKYVLDYRDDEVFWCTADVGWVTGHSYI 313 Query: 317 LYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTD 376 +YGPLA GATTL+FEGVPN+P+ +R QV+DKH+VNI YTAPTA+RALM EG + ++ T Sbjct: 314 VYGPLANGATTLIFEGVPNYPSTSRFWQVIDKHKVNIFYTAPTALRALMREGPEPLKQTS 373 Query: 377 RSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGS 436 R+S+R+LG+VGEPINPEAWEWY+ ++G E+CP+VDTWWQTETGG M++PL A +K G Sbjct: 374 RASVRLLGTVGEPINPEAWEWYFHEVGEERCPIVDTWWQTETGGIMLSPLVSAQRIKPGC 433 Query: 437 ATRPFFGVQPALVDNEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMY 496 AT+P FGVQP L+D +G ++GA G L I SWP Q R+++ D +R TYF + Y Sbjct: 434 ATQPMFGVQPVLLDEQGKEIKGAGSGVLAIKSSWPAQIRSVYRDPQRMVDTYFKPYPGYY 493 Query: 497 FSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKG 556 F+GDGARRDEDG YWITGR+DDV+NVSGHR+GTAE+ESALV H IAEAA VG PH+IKG Sbjct: 494 FTGDGARRDEDGDYWITGRIDDVINVSGHRIGTAEVESALVLHENIAEAAAVGYPHDIKG 553 Query: 557 QAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILR 616 Q IY +VT +G E + EL E+ V KEIG A PD++ W +LPKTRSGKIMRRILR Sbjct: 554 QGIYVFVTPMNGVETNDELKKELLALVSKEIGSFAKPDLIQWAPALPKTRSGKIMRRILR 613 Query: 617 KIAAGDTSNLGDTSTLADPGVVEKLLEEK 645 KIA + NLGDTSTLADP VV+ L++++ Sbjct: 614 KIACNELDNLGDTSTLADPSVVQGLIDKR 642 Lambda K H 0.317 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1354 Number of extensions: 46 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 652 Length of database: 645 Length adjustment: 38 Effective length of query: 614 Effective length of database: 607 Effective search space: 372698 Effective search space used: 372698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory