Align Putrescine transport system permease protein PotI (characterized)
to candidate PfGW456L13_3603 Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1)
Query= SwissProt::P0AFL1 (281 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3603 Length = 275 Score = 162 bits (411), Expect = 6e-45 Identities = 92/241 (38%), Positives = 144/241 (59%), Gaps = 12/241 (4%) Query: 20 FTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAA 79 + FLYAP+ ++V++SFN+ + + S +W+G + +M A+G S IA +A A Sbjct: 11 YLFLYAPIALIVLFSFNAGRSGLAFQCCSVQWFGTAFGNPFIMEALGNSALIAFSSAVIA 70 Query: 80 AILGTIAAVVLVRFGRFRGSNGFAFMITAPLVMPDVITGLSLLLLFVALAHAIG-----W 134 + GT+A L R G + F + +++P ++ G+S L+ F++L I W Sbjct: 71 TLFGTLAVFGLQRCGA-KVRMLFDALTYCAIIVPGIVIGISTLIAFISLFDLINPLLASW 129 Query: 135 -----PADRGMLTIWLAHVTFCTAYVAVVISSRLRELDRSIEEAAMDLGATPLKVFFVIT 189 + G T+ AH F A V V++ SR+ LD+++ EA+ DL A P+ F+ +T Sbjct: 130 LPTVPKLNMGFFTVIAAHSLFTMALVMVIVRSRVDSLDKALLEASADLYAPPMDTFWRVT 189 Query: 190 LPMIMPAIISGWLLAFTLSLDDLVIASFVSGPGATTLPMLVFSSVRMGVNPEINALATLI 249 LP I PAI++G+LLAFT S DD +IA FV+G TTLP+ +FSS+R GV PEINA++T+I Sbjct: 190 LPQISPAIMAGFLLAFTFSFDDFIIAFFVAG-SETTLPIYIFSSIRRGVTPEINAISTVI 248 Query: 250 L 250 + Sbjct: 249 I 249 Lambda K H 0.330 0.140 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 192 Number of extensions: 10 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 281 Length of database: 275 Length adjustment: 25 Effective length of query: 256 Effective length of database: 250 Effective search space: 64000 Effective search space used: 64000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory