Alignments for a candidate for potD in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for potD in Pseudomonas fluorescens GW456-L13

Align Putrescine-binding periplasmic protein SpuD (characterized)
to candidate PfGW456L13_736 Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)

Query= SwissProt::Q02UB7
         (367 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_736
          Length = 366

 Score =  442 bits (1137), Expect = e-129
 Identities = 213/361 (59%), Positives = 271/361 (75%), Gaps = 1/361 (0%)

Query: 7   KTLLALTLAGSVAGMAQAADNKVLHVYNWSDYIAPDTLEKF-TKETGIKVVYDVYDSNEV 65
           K  +A  L  ++ G A  A+ + L VYNW DYI P  LE F  + T  K+VYD++D+NE 
Sbjct: 5   KRFIAPALCATLLGGAVHAEERTLRVYNWFDYITPKALEDFKAQNTQTKLVYDIFDTNEA 64

Query: 66  LEAKLLAGKSGYDVVVPSNSFLAKQIKAGVYQKLDKSKLPNWKNLNKDLMHTLEVSDPGN 125
           LEAKLL G SGYDVVVPSN FLAKQI+AGV+Q LD+SKLPNW +L+  LM  +E +DPGN
Sbjct: 65  LEAKLLTGNSGYDVVVPSNVFLAKQIEAGVFQPLDRSKLPNWNHLDPKLMKLIEANDPGN 124

Query: 126 EHAIPYMWGTIGIGYNPDKVKAAFGDNAPVDSWDLVFKPENIQKLKQCGVSFLDSPTEIL 185
           + A+PYM+GTI IG+NPDKVKAA G +APVDSWDL+FK ENI KLKQCGV+ LDSP+EIL
Sbjct: 125 KFAVPYMYGTILIGFNPDKVKAALGADAPVDSWDLIFKEENISKLKQCGVALLDSPSEIL 184

Query: 186 PAALHYLGYKPDTDNPKELKAAEELFLKIRPYVTYFHSSKYISDLANGNICVAIGYSGDI 245
           P AL +LG  P++  P +   AE L +KIRPY+TYFHSSKY++D+ANG+ICVA+GYSG  
Sbjct: 185 PLALQHLGLDPNSKKPADYDKAEALLMKIRPYITYFHSSKYMADIANGDICVAVGYSGSF 244

Query: 246 YQAKSRAEEAKNKVTVKYNIPKEGAGSFFDMVAIPKDAENTEGALAFVNFLMKPEIMAEI 305
            QA +RA+EAKN V V   +PKEGA  +FDM+AIPK A+N E A  F+N+L++P+++A +
Sbjct: 245 SQAANRAKEAKNGVVVDMRLPKEGAPIWFDMLAIPKGAKNPEDAYTFINYLLQPQVIAPV 304

Query: 306 TDVVQFPNGNAAATPLVSEAIRNDPGIYPSEEVMKKLYTFPDLPAKTQRAMTRSWTKIKS 365
           +D V +PN N  AT LV  AIRN+P +YP++  M  LYT   LP   +RA TR+WTKIKS
Sbjct: 305 SDFVGYPNPNKDATELVDPAIRNNPNLYPTDAAMGTLYTLQPLPRDAERARTRAWTKIKS 364

Query: 366 G 366
           G
Sbjct: 365 G 365


Lambda     K      H
   0.315    0.133    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 481
Number of extensions: 22
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 367
Length of database: 366
Length adjustment: 30
Effective length of query: 337
Effective length of database: 336
Effective search space:   113232
Effective search space used:   113232
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources