Aligments for a candidate for ptsG-crr in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Aligments for a candidate for ptsG-crr in Pseudomonas fluorescens GW456-L13

Align PTS system glucose-specific EIICBA component; EIICBA-Glc; EII-Glc; EIICBA-Glc 1; EC 2.7.1.199 (characterized)
to candidate PfGW456L13_4833 PTS system, N-acetylglucosamine-specific IIA component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component (EC 2.7.1.69)

Query= SwissProt::Q57071
         (675 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4833
          Length = 571

 Score =  365 bits (936), Expect = e-105
 Identities = 216/506 (42%), Positives = 304/506 (60%), Gaps = 52/506 (10%)

Query: 1   MWKKFFGQLQRIGKALMLPVAILPAAGLLLALGNAFQGDALQSLMPFIKAEGFQNVAKMM 60
           M++ F   LQR+G+ALMLP+AILP AGLLL LG+    D L             N+A ++
Sbjct: 1   MYQLFIEGLQRLGRALMLPIAILPIAGLLLRLGDT---DLL-------------NIA-II 43

Query: 61  EGAGGIIFDNLAIIFALGVAIGLA-SGDGVAAIAAFVGFIVLNKTMGMFLGVTPEKAADA 119
             AG +IF NLA+IFA+G+A+G A   +G A +A  +G++V+  T+         KA D 
Sbjct: 44  HDAGQVIFANLAMIFAIGIAVGFARDNNGTAGLAGVIGYLVMISTL---------KALD- 93

Query: 120 ATGFANVLGIPTLQTGVFGGIIIGALAAWCYNKFYNISLPSYLGFFAGKRFVPIMMATCS 179
                     P++  G+  GI+ G +A   YN+F +I LP YL FF G+RFVPI+    +
Sbjct: 94  ----------PSINMGMLAGIVSGLMAGALYNRFKDIKLPEYLAFFGGRRFVPIVTGFAA 143

Query: 180 FILAFPMAIIWPSIQGGLNAFSEGLLASNTGLAVFLFGFIKRLLIPFGLHHIFHAPFWFE 239
             L      IWP IQ G+N+F   ++ S +    F+FG   RLLI  GLHHI +   WF 
Sbjct: 144 VGLGVVFGYIWPPIQHGINSFGTLMMESGS-FGAFVFGLFNRLLIVTGLHHILNNMAWFV 202

Query: 240 FGSYKN-AAGQIIHGD-QRIFIEQIRDNVPLTAGKFMQGEFPVMMFGLPAAALAIYQTAK 297
           FG++ +   G ++ GD  R F    +       G+FM G FP+M+FGLPAA LA+Y+ A 
Sbjct: 203 FGNFTDPTTGALVTGDLSRYFAGDPK------GGQFMTGMFPMMIFGLPAACLAMYRNAL 256

Query: 298 KENKKVVAGLMLSGALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFLILYLLDLHL 357
              +KV+ G+ LS ALT+FLTG+TEP+EF+F+F+APLLF +HA+L GLS  I   L++HL
Sbjct: 257 PARRKVMGGIFLSMALTAFLTGVTEPIEFAFMFLAPLLFVLHALLTGLSMAITNALNIHL 316

Query: 358 GYTFSGGFIDFFLLGILPNKTQWWLVIPVGLVYAAIYYIIFRFLIVKFNFKTPGRE-DKE 416
           G+TFSGGFID  +LG     T  WLV PVGL YA IYY++F F I +F+ KTPGRE   +
Sbjct: 317 GFTFSGGFID-MILG-WGKSTNGWLVFPVGLAYAVIYYVVFDFCIRRFDLKTPGRETSAD 374

Query: 417 VKSSNVAASELPFKVLDAMGGKANIKHLDACITRLRVEVNDKAKVDVQELKDLGASGVLE 476
           V+   VA +E     + A+GG  N+  + AC TRLR+++ D+ K    +LK LGA  V+ 
Sbjct: 375 VEQVAVADNERAGAYIKALGGAENLITVGACTTRLRLDMVDRNKASDTDLKALGAMAVVR 434

Query: 477 --VGNNMQAIFGPKSDQIKHDMQQIM 500
              G ++Q + GP +D I  +++  M
Sbjct: 435 PGKGGSLQVVVGPMADAIADEIRLAM 460



 Score = 34.3 bits (77), Expect = 2e-05
 Identities = 20/72 (27%), Positives = 40/72 (55%), Gaps = 2/72 (2%)

Query: 432 LDAMGGKANIKHLDA-CITRLRVEVNDKAKVDVQELKDLGASGVLEV-GNNMQAIFGPKS 489
           L+A+GG  N+  LD   +TR+R+++ D   +   +LK LG  GV ++ G     + G K+
Sbjct: 492 LNALGGGDNVLQLDCVAMTRIRLQLADGKALSEFDLKALGCQGVSQLDGGIWHLLIGDKA 551

Query: 490 DQIKHDMQQIMD 501
             +   ++ +++
Sbjct: 552 PTLSDALEVLVN 563


Lambda     K      H
   0.324    0.143    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 997
Number of extensions: 57
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 675
Length of database: 571
Length adjustment: 37
Effective length of query: 638
Effective length of database: 534
Effective search space:   340692
Effective search space used:   340692
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources