GapMind for catabolism of small carbon sources

 

Alignments for a candidate for mmsA in Pseudomonas fluorescens GW456-L13

Align methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized)
to candidate PfGW456L13_1083 Betaine aldehyde dehydrogenase (EC 1.2.1.8)

Query= BRENDA::P42412
         (487 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1083
          Length = 490

 Score =  266 bits (679), Expect = 2e-75
 Identities = 170/484 (35%), Positives = 262/484 (54%), Gaps = 12/484 (2%)

Query: 1   MAEIRKLKNYINGEWVESKTDQYEDVVNPATKEVLCQVPISTKEDIDYAAQTAAEAFKTW 60
           MA     K YI+G + ++ +D   + +NPA  EVL +V  +TKED++ A  +A +  K W
Sbjct: 1   MARFELQKLYIDGAYSDASSDATFEAINPANGEVLAKVQRATKEDVERAVVSAEKGQKIW 60

Query: 61  SKVAVPRRARILFNFQQLLSQHKEELAHLITIENGKNTKEA-LGEVGRGIENVEFAAGAP 119
           + +    R+RIL    ++L +  +ELA L T++ GK   E    ++  G + +E+ AG  
Sbjct: 61  AAMTAMERSRILRRAVEILRERNDELAALETLDTGKAYSETRYVDIVTGADVLEYYAGLV 120

Query: 120 SLMMGDSLASIATDVEAANYRYPIGVVGGIAPFNFPMMVPCWMFPMAIALGNTFILKPSE 179
             + G+ +  + T       R P+GVV GI  +N+P+ +  W    A+A GN  I KPSE
Sbjct: 121 PAIEGEQIP-LRTTSFVYTRREPLGVVAGIGAWNYPIQIALWKSAPALAAGNAMIFKPSE 179

Query: 180 RTPLLTEKLVELFEKAGLPKGVFNVVYGA-HDVVNGILEHPEIKAISFVGSKPVGEYVY- 237
            T L T KL E++ +AG+P GVFNV+ G+  +V   + EHP I+ +SF G    G+ V  
Sbjct: 180 VTSLTTLKLAEIYTEAGVPNGVFNVLTGSGREVGTWLTEHPRIEKVSFTGGTDTGKKVMA 239

Query: 238 KKGSENLKRVQSLTGAKNHTIVLNDANLEDTVTNIVGAAFGSAGERCMACAVVTVEEGIA 297
              S +LK V    G K+  I+ +DA+L+      + A F S+G+ C     V V   + 
Sbjct: 240 SASSSSLKDVTMELGGKSPLIICDDADLDRAADTAMMANFYSSGQVCTNGTRVFVPAHLK 299

Query: 298 DEFMAKLQEKVADIKIGNGLDDGVFLGPVIREDNKKRTLSYIEKGLEEGARLVCDGRENV 357
             F AK+ E+VA I+IGN  D+    GP++   + +  L YI KG EEGARL+C G E +
Sbjct: 300 AAFEAKIAERVARIRIGNPEDENTNFGPLVSFAHMESVLGYIAKGKEEGARLLCGG-ERL 358

Query: 358 SD----DGYFVGPTIFDNVTTEMTIWKDEIFAPVLSVIRVKNLKEAIEIANKSEFANGAC 413
           +D     G FV PT+F + T +MTI ++EIF PV++++  +  +E I  AN ++F   A 
Sbjct: 359 TDGEFAKGAFVAPTVFTDCTDDMTIVREEIFGPVMAILSYETEEEVIRRANDTDFGLAAG 418

Query: 414 LFTSNSNAIRYFRENIDAGMLGINLGVPAPMAFFPFSGWKSSFFGTLHANGKDSVDFYTR 473
           + T + N        ++AG+  IN    +  A  P  G+K S  G    NG  S++ +TR
Sbjct: 419 IVTKDLNRAHRVIHQLEAGICWINAWGESD-AKMPVGGYKQS--GVGRENGISSLNNFTR 475

Query: 474 KKVV 477
            K V
Sbjct: 476 IKSV 479


Lambda     K      H
   0.318    0.136    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 575
Number of extensions: 23
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 487
Length of database: 490
Length adjustment: 34
Effective length of query: 453
Effective length of database: 456
Effective search space:   206568
Effective search space used:   206568
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory