GapMind for catabolism of small carbon sources

 

Finding step natE for L-valine catabolism in Pseudomonas fluorescens GW456-L13

4 candidates for natE: L-valine ABC transporter, ATPase component 2 (NatE)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
med PfGW456L13_4611 Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1) NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 50% 94% 228.4 High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine 87% 391.3
med PfGW456L13_120 Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1) NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 44% 96% 189.9 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.8
lo PfGW456L13_876 Glutamate transport ATP-binding protein NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 32% 95% 115.2 Amino acid ABC transporter ATP binding protein, component of Hydroxy L-proline uptake porter, HprABC 56% 265.0
lo PfGW456L13_4204 ABC transporter, ATP-binding protein NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 84% 114 spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 46% 271.6

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step natE

Or cluster all characterized natE proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory