Alignments for a candidate for fruI in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruI in Pseudomonas fluorescens GW456-L13

Align The fructose inducible fructose/xylitol porter, FruI (characterized)
to candidate PfGW456L13_5073 PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)

Query= TCDB::Q1LZ59
         (655 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5073
          Length = 579

 Score =  341 bits (875), Expect = 5e-98
 Identities = 212/547 (38%), Positives = 310/547 (56%), Gaps = 52/547 (9%)

Query: 115 AALAELSQYLLKDGFADKLRQVTNPDDVINLFNATEEEKKEATPAA--PVA--DSHDFLV 170
           +A  E+S+++ K  F     Q     + +    A + +   A  AA  PVA   +   LV
Sbjct: 64  SAPVEMSRFVGKRVFQSTPAQALQDVEAVLRRGAQDAQVYAAEEAAVEPVAVVQNAPRLV 123

Query: 171 AVTACTTGIAHTYMAEEALKKQAAEMGVGIKVETNGASGVGNKLTADDIKRAKGVIIAAD 230
           AVTAC TG+AHT+MA EAL++ A  +G  ++VET G+ G  N L+A  I  A  V++AAD
Sbjct: 124 AVTACPTGVAHTFMAAEALQQAAKRLGYELQVETQGSVGARNPLSAQAIAEADVVLLAAD 183

Query: 231 KAVEMDRFNGKPLISRPVAEGIKKPEELINIILDGKAEAYVADNSDLSSEASSSEKAGLG 290
             V  +RF GK +        +K+ E  +   L   AE      S  +   +  EK G+ 
Sbjct: 184 IEVATERFAGKKIYRCGTGIALKQAEATLKKAL---AEGQQESASSAAKTTAKQEKTGV- 239

Query: 291 SAFYKHLMSGVSQMLPFVIGGGIMIALSFLIDQFMGVPKSSLSHLGNYHEIAAIFNQVGN 350
              YKHL++GVS MLP V+ GG+MIALSF+        + +L+        AA+    G+
Sbjct: 240 ---YKHLLTGVSFMLPMVVAGGLMIALSFVFGITAFKEEGTLA--------AALMQIGGD 288

Query: 351 AAFGFMIPVFAAYIAYSIAEKPGLVAGFVAGSMATTGLAFNKVAFFEFGEKASQASLTGI 410
           +AF  M+P+ A YIAYSIA++PGL  G + G +A+T                       +
Sbjct: 289 SAFKLMVPLLAGYIAYSIADRPGLAPGMIGGLLAST-----------------------L 325

Query: 411 PSGFLGALAGGFLAGGVILVLKKALAFVPRSLEGIKSILLYPLLGVLVTGFLMLFV-NIP 469
            +GF+G +  GFLAG     + +  A +P+SLE +K IL+ PLL  L TG +M++V   P
Sbjct: 326 GAGFIGGIIAGFLAGYAARAINR-YARLPQSLEALKPILIIPLLASLFTGLVMIYVVGKP 384

Query: 470 MAAINTALYNFLGNLSGGSAVLLGLIVGGMMAIDMGGPFNKAAYVFGTSTLTAAALAKGG 529
           +A +   L NFL ++   +A+LLG+++GGMM +D+GGP NKAAY F    L + + A   
Sbjct: 385 VAGMLAGLTNFLDSMGTTNAILLGVLLGGMMCVDLGGPINKAAYAFSVGLLASQSYAP-- 442

Query: 530 SVVMASVMAGGMVPPLAVFVATLLFKNKFTQEEHDAGLTNIVMGLSFITEGAIPFGAGDP 589
              MA+ MA GMVPP+ + +AT + + KF Q E +AG   +V+GL FI+EGAIPF A DP
Sbjct: 443 ---MAATMAAGMVPPIGLGIATFIARRKFAQTEREAGKAALVLGLCFISEGAIPFAAKDP 499

Query: 590 ARAIPSFIVGSAVTGALVGLSGIKLMAPHGGIFVIAL---TSNPLLYLLYIAVGAVIAGI 646
            R IP+ I G A+TGAL    G KLMAPHGG+FV+A+    ++ LLYLL I  G+++  +
Sbjct: 500 LRVIPASIAGGALTGALSMYFGCKLMAPHGGLFVLAIPNAINHALLYLLAIVAGSLLTAV 559

Query: 647 LFGSLRK 653
            +  +++
Sbjct: 560 AYALVKR 566



 Score = 42.7 bits (99), Expect = 5e-08
 Identities = 25/91 (27%), Positives = 41/91 (45%)

Query: 169 LVAVTACTTGIAHTYMAEEALKKQAAEMGVGIKVETNGASGVGNKLTADDIKRAKGVIIA 228
           L  VTAC  G+  + +    L   A   G    VE   A+    +L+A  +  A+ V++ 
Sbjct: 3   LAIVTACPNGMVTSVLCARLLDAAAQRQGWSTSVEVTDAAHPEKQLSAATLDAAEWVLLV 62

Query: 229 ADKAVEMDRFNGKPLISRPVAEGIKKPEELI 259
               VEM RF GK +     A+ ++  E ++
Sbjct: 63  TSAPVEMSRFVGKRVFQSTPAQALQDVEAVL 93


Lambda     K      H
   0.320    0.137    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 824
Number of extensions: 41
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 655
Length of database: 579
Length adjustment: 37
Effective length of query: 618
Effective length of database: 542
Effective search space:   334956
Effective search space used:   334956
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources