GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aldox-small in Pseudomonas fluorescens GW456-L13

Align glycolaldehyde oxidoreductase small subunit (characterized)
to candidate PfGW456L13_4084 Xanthine dehydrogenase iron-sulfur subunit (EC 1.17.1.4) / Xanthine dehydrogenase, FAD binding subunit (EC 1.17.1.4)

Query= metacyc::MONOMER-18073
         (163 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4084
          Length = 484

 Score =  108 bits (269), Expect = 2e-28
 Identities = 51/145 (35%), Positives = 80/145 (55%), Gaps = 13/145 (8%)

Query: 22  ERYVSPRILLVDFLREELGLTGTKIGCDTTTCGACTVLLNG-------------KSVKSC 68
           E  + P + ++++LR+ +G  GTK GC +  CGACTV++               +S+ SC
Sbjct: 13  EHALDPNLTVLNYLRDHVGKPGTKEGCASGDCGACTVVVGELQTDDDGREHIRYRSLNSC 72

Query: 69  TLFAVQADGAEITTIEGLSVDSKLHPIQEAFKENFALQCGFCTPGMIMQAYFLLKENPNP 128
             F     G ++ ++E L    +LH +Q+A  E    QCGFCTPG +M  + L K +  P
Sbjct: 73  LTFVSSLHGKQLISVEDLKHKGELHSVQKAMVECHGSQCGFCTPGFVMSLFALQKNSDAP 132

Query: 129 SEEEVRDGLHGNICRCTGYQNIVKA 153
              +  + L GN+CRCTGY+ I++A
Sbjct: 133 DHAKAHEALAGNLCRCTGYRPILEA 157


Lambda     K      H
   0.322    0.138    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 160
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 163
Length of database: 484
Length adjustment: 25
Effective length of query: 138
Effective length of database: 459
Effective search space:    63342
Effective search space used:    63342
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory