GapMind for catabolism of small carbon sources

 

Protein Pf1N1B4_1640 in Pseudomonas fluorescens FW300-N1B4

Annotation: Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1)

Length: 257 amino acids

Source: pseudo1_N1B4 in FitnessBrowser

Candidate for 7 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-lysine catabolism hisP hi ABC transporter for L-Lysine, ATPase component (characterized) 97% 100% 487.3 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 66% 334.7
L-arginine catabolism artP hi Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 93% 100% 472.6 NocP aka ATU6028 aka AGR_PTI_69, component of Nopaline porter 62% 316.2
L-histidine catabolism hisP hi Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized) 93% 100% 472.6 AotP aka PA0892, component of Arginine/ornithine (but not lysine) porter 66% 334.7
L-histidine catabolism BPHYT_RS24015 med ABC transporter related (characterized, see rationale) 65% 97% 330.5 ABC transporter for L-Lysine, ATPase component 97% 487.3
L-citrulline catabolism AO353_03040 med ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized) 65% 98% 329.3 ABC transporter for L-Lysine, ATPase component 97% 487.3
D-glucosamine (chitosamine) catabolism AO353_21725 med ABC transporter for D-glucosamine, ATPase component (characterized) 53% 94% 260.8 ABC transporter for L-Lysine, ATPase component 97% 487.3
L-histidine catabolism PA5503 lo Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized) 39% 72% 173.7 ABC transporter for L-Lysine, ATPase component 97% 487.3

Sequence Analysis Tools

View Pf1N1B4_1640 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MAEATPALEIRNLHKRYGQLEVLKGISLTARDGDVISILGSSGSGKSTFLRCINLLENPH
QGQILVAGEELKLKAAKNGELVAADGKQINRLRSEIGFVFQNFNLWPHMSVLDNIIEAPR
RVLGQSKAEAIEVAEALLAKVGIADKRHAYPAQLSGGQQQRAAIARTLAMQPKVILFDEP
TSALDPEMVQEVLNVIRALAEEGRTMLLVTHEMSFARQVSSEVVFLHQGLVEEQGSPQQV
FENPLSARCKQFMSSNR

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory