GapMind for catabolism of small carbon sources


2-oxoglutarate (alpha-ketoglutarate) catabolism in Pseudomonas fluorescens FW300-N1B4

Best path


Also see fitness data for the top candidates


Overview: Since 2-oxoglutarate (also known as alpha-ketoglutarate) is a TCA cycle intermediate, GapMind represents uptake only.

9 steps (1 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
kgtP 2-oxoglutarate:H+ symporter KgtP Pf1N1B4_686 Pf1N1B4_5606
Alternative steps:
csbX 2-oxoglutarate permease csbX
dctM 2-oxoglutarate TRAP transporter, large permease component DctM
dctP 2-oxoglutarate TRAP transporter, solute receptor component DctP
dctQ 2-oxoglutarate TRAP transporter, small permease component DctQ
Dshi_1194 TRAP transporter for succinate, fumarate, L-malate, and 2-oxoglutarate, fused 4TM/12TM components
Dshi_1195 TRAP transporter for succinate, fumarate, L-malate, and 2-oxoglutarate, substrate-binding component
Psest_0084 2-oxoglutarate TRAP transporter, fused 4TM/12TM components
Psest_0085 2-oxoglutarate TRAP transporter, solute receptor component

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory