GapMind for catabolism of small carbon sources

 

Alignments for a candidate for glcD in Pseudomonas fluorescens FW300-N1B4

Align D-lactate oxidase and glycolate oxidase, FAD-linked subunit (EC 1.1.3.15) (characterized)
to candidate Pf1N1B4_5862 Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD

Query= reanno::psRCH2:GFF3772
         (499 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5862
          Length = 499

 Score =  888 bits (2295), Expect = 0.0
 Identities = 431/499 (86%), Positives = 470/499 (94%)

Query: 1   MNILYDERVDGALPKVDKAALLAELQAQLPDLDILHRSEDLKPYECDGLSAYRTTPLLVV 60
           MNILYDER+DGALP VDKAALL  LQ ++PDLDIL R E+LKPYECDGLSAYRTTP+LV 
Sbjct: 1   MNILYDERIDGALPNVDKAALLKALQQEVPDLDILWREEELKPYECDGLSAYRTTPMLVA 60

Query: 61  LPERIEQVETLLKLCHQRGVPVVARGAGTGLSGGALPLEQGILLVMARFNKILEVDPAGR 120
           LP ++EQV+ LLKLCH R VPVVARGAGTGLSGGALPL++G+LLVM RFN IL +DPA R
Sbjct: 61  LPRQLEQVQALLKLCHARNVPVVARGAGTGLSGGALPLDKGVLLVMVRFNNILHIDPAAR 120

Query: 121 FARVQPGVRNLAISQAAAPYELYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL 180
            ARVQPGVRNLAISQAAAP+ LYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
Sbjct: 121 TARVQPGVRNLAISQAAAPFGLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL 180

Query: 181 KVDILTVEGERMTLGSDALDSPGFDLLALFTGSEGMLGIVTEVTVKLLPKPQVAKVLLAA 240
           K+++LT+EGE +TLG+D+LD+PGFDLLALFTGSEG+LGI+TEVTVKLLPKPQVAKVLLA+
Sbjct: 181 KLEVLTLEGEHLTLGADSLDAPGFDLLALFTGSEGLLGIITEVTVKLLPKPQVAKVLLAS 240

Query: 241 FDSVEKAGRAVGDIIAAGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVEA 300
           FDSVEKAGR V +IIAAGIIPGGLEMMDNL+IRAAEDFIHAGYPVDAEAILLCELDGVEA
Sbjct: 241 FDSVEKAGRTVAEIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVDAEAILLCELDGVEA 300

Query: 301 DVHDDCARVSEVLKLAGATEVRLAKDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPR 360
           DVHDDC RV EVL+ AGA++VR A+DEAERVRFWAGRKNAFPA+GR+SPDYYCMDGTIPR
Sbjct: 301 DVHDDCERVREVLQQAGASDVRQARDEAERVRFWAGRKNAFPAIGRLSPDYYCMDGTIPR 360

Query: 361 RELPGVLKGISDLSEQFGLRVANVFHAGDGNMHPLILFDANQPGELERAEDLGGKILELC 420
           RELPGVLKGI+ L++++GLRVANVFHAGDGNMHPLILFDANQPGELERAE LGGKILELC
Sbjct: 361 RELPGVLKGIASLAQEYGLRVANVFHAGDGNMHPLILFDANQPGELERAEALGGKILELC 420

Query: 421 VKVGGSITGEHGVGREKINQMCSQFNADELTLFHAVKAAFDPSGLLNPGKNIPTLHRCAE 480
           VKVGGSITGEHGVGREKINQMC+QFN+DELTLFHAVKAAFDP GLLNPGKNIPTLHRCAE
Sbjct: 421 VKVGGSITGEHGVGREKINQMCAQFNSDELTLFHAVKAAFDPKGLLNPGKNIPTLHRCAE 480

Query: 481 FGRMHIHNGQLPFPELERF 499
           FG MHIH GQLPFPELERF
Sbjct: 481 FGAMHIHAGQLPFPELERF 499


Lambda     K      H
   0.320    0.140    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 957
Number of extensions: 19
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 499
Length of database: 499
Length adjustment: 34
Effective length of query: 465
Effective length of database: 465
Effective search space:   216225
Effective search space used:   216225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory