Align Acetate/haloacid transporter, Dehp2, with a possible atypical topology (characterized)
to candidate Pf1N1B4_5961 Major facilitator superfamily transporter
Query= TCDB::F8SVK1 (552 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5961 Length = 558 Score = 785 bits (2028), Expect = 0.0 Identities = 382/550 (69%), Positives = 460/550 (83%), Gaps = 3/550 (0%) Query: 3 TVSGQISHAPMTKEEKRVIFASSLGTVFEWYDFYLAGSLAAFISKSFFSGVNPTAAFIFT 62 T +G H +T+EE++VIFASSLGTVFEWYDFYL GSLAA I+K FF+GVN T +FIF Sbjct: 7 TTTGSAPHHGITREERKVIFASSLGTVFEWYDFYLYGSLAAIIAKHFFAGVNETTSFIFA 66 Query: 63 LLGFAAGFAVRPFGALVFGRLGDMVGRKYTFLITIVIMGLSTCVVGFLPGYAAIGMASPV 122 LL FAAGFAVRPFGA+VFGRLGDM+GRK+TFLITIVIMG+ST VVGFLPGYA IG+A+P+ Sbjct: 67 LLAFAAGFAVRPFGAIVFGRLGDMIGRKHTFLITIVIMGISTAVVGFLPGYATIGVAAPI 126 Query: 123 IFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGVRT 182 I I +RLLQGLALGGEYGGAATYVAEHAP +RG++T+WIQTTATLGLFLSLLVIL RT Sbjct: 127 ILITLRLLQGLALGGEYGGAATYVAEHAPKGKRGYFTSWIQTTATLGLFLSLLVILACRT 186 Query: 183 AMGEDAFGAWGWRIPFVASLVLLGISVWIRMQLHESPAFERIKAEGKTSKAPLSEAFGQW 242 +G +AF AWGWRIPF+ S++LL +SV+IR+QL ESP F ++K EGK+SKAPL+E+F +W Sbjct: 187 ILGNEAFEAWGWRIPFLLSILLLIVSVYIRLQLSESPVFLKMKEEGKSSKAPLTESFARW 246 Query: 243 KNLKIVILALIGVTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLF 302 +NLKIVI+AL+G TAGQAVVWYTGQFYALFFL QTLK+D +AN LIA +LLIGTPFF+ Sbjct: 247 ENLKIVIMALLGGTAGQAVVWYTGQFYALFFLLQTLKIDPQTANFLIAGSLLIGTPFFII 306 Query: 303 FGSLSDRIGRKPIILAGCLIAALTYFPLFKALTHYANPALEAATQKSPIVVIANPDECSF 362 FGSLSDRIGRK II+AGC+IAALTYFP+F ALT Y NP + AA +K+P+ VIA P +CSF Sbjct: 307 FGSLSDRIGRKGIIMAGCVIAALTYFPIFHALTEYGNPDVFAAQEKNPVTVIAAPGQCSF 366 Query: 363 QFNPVGTSKFTSSCDIAKSALSKAGLNYDNVAAPAGTLAQIKVGDTTIDTYDG---KAAD 419 QF+PVG +KFTSSCD+AK+ L+K + Y+NV A G +AQ+++GD I +++G AAD Sbjct: 367 QFDPVGKAKFTSSCDLAKTVLAKKAIPYENVKAEPGAIAQVRIGDKVIQSFEGTGMPAAD 426 Query: 420 AKDAGKAFDKNLGTALKAASYPPKADPSQLNWPMTVVILTILVIYVTMVYGPIAAMLVEM 479 K AF LGTALK A YP KADP++ N+PM +++LTILVIYVTMVYGPIAA LVE+ Sbjct: 427 FKARNDAFVATLGTALKDAGYPEKADPAKTNYPMVLLLLTILVIYVTMVYGPIAAWLVEL 486 Query: 480 FPTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIIIALATFVIGLLF 539 FP RIRYTSMSLPYHIGNGWFGGFLP AFA+VAA G+IY GLWYPI+IA+ T ++G F Sbjct: 487 FPARIRYTSMSLPYHIGNGWFGGFLPTVAFAMVAATGDIYYGLWYPIVIAVMTAILGTFF 546 Query: 540 VRETKDSNIY 549 + ETKD I+ Sbjct: 547 LPETKDREIH 556 Lambda K H 0.325 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 918 Number of extensions: 40 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 558 Length adjustment: 36 Effective length of query: 516 Effective length of database: 522 Effective search space: 269352 Effective search space used: 269352 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory