Align ABC transporter related (characterized, see rationale)
to candidate Pf1N1B4_4286 Inositol transport system ATP-binding protein
Query= uniprot:A0KWY5 (499 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4286 Length = 526 Score = 358 bits (919), Expect = e-103 Identities = 191/492 (38%), Positives = 310/492 (63%), Gaps = 7/492 (1%) Query: 4 ILELKQISKHYPGVKALEDVSLRLFAGEVHALLGENGAGKSTLVKVMTGAQSKDMGDILF 63 +LE+ +SK +PGV AL DV LR+ G V AL+GENGAGKSTL+K++ G D G++ Sbjct: 32 LLEIINVSKGFPGVVALSDVQLRVRPGSVLALMGENGAGKSTLMKIIAGIYQPDAGELRL 91 Query: 64 LGEPQHFNTPMDAQKAGISTVYQEVNLVPNLTVAQNLFLGYEPRR-LGLIHFKKMYADAR 122 G+P F TP+ A +AGI+ ++QE+NL+P++++A+N+++G E L +I ++M+ Sbjct: 92 RGKPVVFETPLAALQAGIAMIHQELNLMPHMSIAENIWIGREQLNGLHMIDHREMHRCTA 151 Query: 123 AVLTQFKLDIDVSAPLSDYSIAVQQLIAIARGVAMSAKVLVLDEPTASLDAKEVQVLFGI 182 +L + ++++D + + SIA +Q++ IA+ V+ + +L++DEPT+++ KEV LF I Sbjct: 152 KLLERLRINLDPEELVGNLSIAERQMVEIAKAVSYDSDILIMDEPTSAITDKEVAHLFSI 211 Query: 183 LNQLKAKGVAIVFITHFLDQVYQISDRITVLRNGQFIGEYLTAELPQPKLIEAMLGRSLQ 242 + LK +G I++ITH +++V+ I+D + V R+G +IG + LI M+GR L Sbjct: 212 IADLKRQGKGIIYITHKMNEVFSIADEVAVFRDGAYIGLQRADSMDGDSLISMMVGRELS 271 Query: 243 EQLVDKQEKERTVTRAEAVLLSLEDVSVKGSIQSMNLTVPKGQAVGLAGLLGSGRSEVCN 302 QL +EK +LLS+ D+ + G + ++ + G+ +G+AGL+GSGR+ V Sbjct: 272 -QLFPVREKP-----IGDLLLSVRDLKLDGIFKDVSFDLHAGEILGIAGLMGSGRTNVAE 325 Query: 303 AVFGLDLVDSGSIHLAGQKLNLSQPVDAISAGIALCPEDRKIDGIIGPLSIRENIILALQ 362 A+FG+ D G I L G+ + +S P AI G AL EDRK+ G+ LS+ EN+ +A+ Sbjct: 326 AIFGITPSDGGEIRLDGEVVRISDPHMAIEKGFALLTEDRKLSGLFPCLSVLENMEMAVL 385 Query: 363 ARIGWWRYLSNTRQQEIAQFFIDKLQIATPDADKPIEQLSGGNQQKVILARWLAIEPILL 422 ++ + + + KL++ TP ++ I+ LSGGNQQK +LARWL P +L Sbjct: 386 PHYVGNGFIQQKALRALCEDMCKKLRVKTPSLEQCIDTLSGGNQQKALLARWLMTNPRIL 445 Query: 423 VLDEPTRGIDIGAHAEIVKLIRTLCDEGMSLLVASSELDELVAFSNKVVVLRDRYAVREL 482 +LDEPTRGID+GA AEI +LI L EGM++++ SSEL E++ S++V+V+ + + L Sbjct: 446 ILDEPTRGIDVGAKAEIYRLISYLASEGMAVIMISSELPEVLGMSDRVMVMHEGDLMGTL 505 Query: 483 SGAELTSQHVMQ 494 +E T + VMQ Sbjct: 506 DRSEATQERVMQ 517 Score = 84.7 bits (208), Expect = 7e-21 Identities = 60/230 (26%), Positives = 113/230 (49%), Gaps = 14/230 (6%) Query: 21 EDVSLRLFAGEVHALLGENGAGKSTLVKVMTGAQSKDMGDILFLGEPQHFNTPMDAQKAG 80 +DVS L AGE+ + G G+G++ + + + G D G+I GE + P A + G Sbjct: 298 KDVSFDLHAGEILGIAGLMGSGRTNVAEAIFGITPSDGGEIRLDGEVVRISDPHMAIEKG 357 Query: 81 ISTVYQE---VNLVPNLTVAQNLFLGYEPRRLG--LIHFKKMYADARAVLTQFKLDIDVS 135 + + ++ L P L+V +N+ + P +G I K + RA+ + V Sbjct: 358 FALLTEDRKLSGLFPCLSVLENMEMAVLPHYVGNGFIQQKAL----RALCEDMCKKLRVK 413 Query: 136 APLSDYSIAV-----QQLIAIARGVAMSAKVLVLDEPTASLDAKEVQVLFGILNQLKAKG 190 P + I QQ +AR + + ++L+LDEPT +D ++ +++ L ++G Sbjct: 414 TPSLEQCIDTLSGGNQQKALLARWLMTNPRILILDEPTRGIDVGAKAEIYRLISYLASEG 473 Query: 191 VAIVFITHFLDQVYQISDRITVLRNGQFIGEYLTAELPQPKLIEAMLGRS 240 +A++ I+ L +V +SDR+ V+ G +G +E Q ++++ G S Sbjct: 474 MAVIMISSELPEVLGMSDRVMVMHEGDLMGTLDRSEATQERVMQLASGMS 523 Lambda K H 0.319 0.136 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 584 Number of extensions: 30 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 499 Length of database: 526 Length adjustment: 35 Effective length of query: 464 Effective length of database: 491 Effective search space: 227824 Effective search space used: 227824 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory