GapMind for catabolism of small carbon sources

 

Alignments for a candidate for xacD in Pseudomonas fluorescens FW300-N1B4

Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate Pf1N1B4_4621 L-arabonate dehydratase (EC 4.2.1.25)

Query= reanno::pseudo5_N2C3_1:AO356_24585
         (578 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4621
          Length = 578

 Score = 1147 bits (2968), Expect = 0.0
 Identities = 565/578 (97%), Positives = 575/578 (99%)

Query: 1   MSDKKPSLRSAQWFGTADKNGFMYRSWMKNQGIADHQFHGKPIIGICNTWSELTPCNAHF 60
           MSDKKP+LRSAQWFGTADKNGFMYRSWMKNQGIADHQFHGKPIIGICNTWSELTPCNAHF
Sbjct: 1   MSDKKPTLRSAQWFGTADKNGFMYRSWMKNQGIADHQFHGKPIIGICNTWSELTPCNAHF 60

Query: 61  RQIAEHVKRGVIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEEAIRGNPIDGVVL 120
           RQIAEHVKRGVIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEEAIRGNPIDGVVL
Sbjct: 61  RQIAEHVKRGVIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEEAIRGNPIDGVVL 120

Query: 121 LTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHKGQDIGSGTVVWQLSEQVKAGTITI 180
           LTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHKGQDIGSGTVVWQLSEQVKAGTIT+
Sbjct: 121 LTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHKGQDIGSGTVVWQLSEQVKAGTITL 180

Query: 181 DDFLAAEGGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDARRYVLAHMSGMR 240
           DDFLAAEGGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDARRYVLAHMSGMR
Sbjct: 181 DDFLAAEGGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDARRYVLAHMSGMR 240

Query: 241 AVEMVREDLKLSKILTKEAFENAIRVNAAIGGSTNAVIHLKAIAGRIGVQLDLDDWTRIG 300
           AVEMVREDLKLSKILTKEAFENAIRVNAAIGGSTNAVIHLKAIAGRIGV+LDLDDW+RIG
Sbjct: 241 AVEMVREDLKLSKILTKEAFENAIRVNAAIGGSTNAVIHLKAIAGRIGVELDLDDWSRIG 300

Query: 301 RGMPTIVDLQPSGRFLMEEFYYAGGLPAVLRRLGEANLIPNPNALTVNGKSLGENTKDAP 360
           RGMPTIVDLQPSGRFLMEEFYYAGGLPAVLRRLGEANLIPNPNALTVNGK++GENTKDAP
Sbjct: 301 RGMPTIVDLQPSGRFLMEEFYYAGGLPAVLRRLGEANLIPNPNALTVNGKTIGENTKDAP 360

Query: 361 IYGQDEVIRTLDNPIRADGGICVLRGNLAPLGAVLKPSAATAELMQHRGRAVVFENFDEY 420
           IYGQDEVIRTLDNPIRADGGICVLRGNLAPLGAVLKPSAA+AELMQHRGRAVVFENFD Y
Sbjct: 361 IYGQDEVIRTLDNPIRADGGICVLRGNLAPLGAVLKPSAASAELMQHRGRAVVFENFDMY 420

Query: 421 KARINDPELDVDANSILVMKNCGPKGYPGMAEVGNMGLPAKLLAQGVTDMVRISDARMSG 480
           KARINDPELDVDANSILVMKNCGPKGYPGMAEVGNMGLPAKLLAQGVTDMVRISDARMSG
Sbjct: 421 KARINDPELDVDANSILVMKNCGPKGYPGMAEVGNMGLPAKLLAQGVTDMVRISDARMSG 480

Query: 481 TAYGTVVLHVAPEAAAGGPLAAVKEGDWIELDCASGRLHLDIPDAELAARLADLAPPQQL 540
           TAYGTVVLHVAPEAAAGGPLA VKEGDWIELDCA+GRLHLDIPDAELAAR+ADL PPQQL
Sbjct: 481 TAYGTVVLHVAPEAAAGGPLATVKEGDWIELDCANGRLHLDIPDAELAARMADLQPPQQL 540

Query: 541 LVGGYRQLYIDHVLQADQGCDFDFLVGCRGAEVPRHSH 578
           +VGGYRQLYIDHVLQADQGCDFDFLVGCRGAEVPRHSH
Sbjct: 541 IVGGYRQLYIDHVLQADQGCDFDFLVGCRGAEVPRHSH 578


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1264
Number of extensions: 46
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 578
Length adjustment: 36
Effective length of query: 542
Effective length of database: 542
Effective search space:   293764
Effective search space used:   293764
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory