Align Arginine N-succinyltransferase subunit alpha; ARUAI; EC 2.3.1.109; AOST; AST (uncharacterized)
to candidate Pf1N1B4_3442 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109)
Query= curated2:P80357 (338 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 Length = 339 Score = 574 bits (1479), Expect = e-168 Identities = 289/339 (85%), Positives = 314/339 (92%), Gaps = 1/339 (0%) Query: 1 MLVMRPAQAADLPQVQRLAADSPVGVTSLPDDAERLRDKILASEASFAAEVSYNGEESYF 60 MLVMRPAQ ADL +VQRLAADSP+GVTSLPDD ERL DKI ASEASFAAEVS+NGEESYF Sbjct: 1 MLVMRPAQMADLGEVQRLAADSPIGVTSLPDDVERLSDKIAASEASFAAEVSFNGEESYF 60 Query: 61 FVLEDSASGELVGCSAIVASAGFSEPFYSFRNETFVHASRSLSIHNKIHVLSLCHDLTGN 120 FVLED+A+G+LVGCSAIVASAG+SEPFYSFRNETFVHASR L IHNKIHVLS CHDLTGN Sbjct: 61 FVLEDTATGKLVGCSAIVASAGYSEPFYSFRNETFVHASRELKIHNKIHVLSQCHDLTGN 120 Query: 121 SLLTSFYVQRDLVQSVYAELNSRGRLLFMASHPERFADAVVVEIVGYSDEQGESPFWNAV 180 SLLTSFYV+ +LV S +AELNSRGRLLF+ASHPERFAD+VV EIVGYSDE G+SPFW+A+ Sbjct: 121 SLLTSFYVKPELVGSPWAELNSRGRLLFVASHPERFADSVVTEIVGYSDENGDSPFWDAI 180 Query: 181 GRNFFDLNYIEAEKLSGLKSRTFLAELMPHYPIYVPLLPDAAQESMGQVHPRAQITFDIL 240 GRNFFDLNY AE+L GLKSRTFLAELMPHYPIYVPLLPD+AQE+MGQVHPRAQITFDIL Sbjct: 181 GRNFFDLNYAAAERLCGLKSRTFLAELMPHYPIYVPLLPDSAQEAMGQVHPRAQITFDIL 240 Query: 241 MREGFETDNYIDIFDGGPTLHARTSGIRSIAQSRVVPVKIGEAPK-SGRPYLVTNGQLQD 299 MREGFETD+YIDIFDGGPTLHAR SGIRSIAQSRVVPVKIGE K +GR YLV N QLQD Sbjct: 241 MREGFETDHYIDIFDGGPTLHARVSGIRSIAQSRVVPVKIGEPVKGAGRQYLVANAQLQD 300 Query: 300 FRAVVLDLDWAPGKPVALSVEAAEALGVGEGASVRLVAV 338 +RAV+L+LD+APGKPV L +EAAEALGVGEGASVRLVAV Sbjct: 301 YRAVLLELDYAPGKPVTLDLEAAEALGVGEGASVRLVAV 339 Lambda K H 0.319 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 450 Number of extensions: 9 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 338 Length of database: 339 Length adjustment: 28 Effective length of query: 310 Effective length of database: 311 Effective search space: 96410 Effective search space used: 96410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate Pf1N1B4_3442 (Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109))
to HMM TIGR03245 (aruF: arginine/ornithine succinyltransferase, alpha subunit (EC 2.3.1.109))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03245.hmm # target sequence database: /tmp/gapView.6505.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03245 [M=336] Accession: TIGR03245 Description: arg_AOST_alph: arginine/ornithine succinyltransferase, alpha subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.3e-171 555.3 0.0 3.7e-171 555.2 0.0 1.0 1 lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 Arginine N-succinyltransferase, Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 555.2 0.0 3.7e-171 3.7e-171 1 336 [] 3 339 .] 3 339 .] 0.99 Alignments for each domain: == domain 1 score: 555.2 bits; conditional E-value: 3.7e-171 TIGR03245 1 vvrParladldaierlaresaigvtslPadrekleekiaqsessfaaevsfsgeesylfvleds 64 v+rPa++adl +++rla++s+igvtslP+d e+l +kia+se sfaaevsf+geesy+fvled+ lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 3 VMRPAQMADLGEVQRLAADSPIGVTSLPDDVERLSDKIAASEASFAAEVSFNGEESYFFVLEDT 66 79************************************************************** PP TIGR03245 65 etgkllGtasivasaGyaePfysyrndtlvhasrelkvnnkihvlslsheltGksllasfyvdp 128 +tgkl+G+++ivasaGy+ePfys+rn+t+vhasrelk++nkihvls +h+ltG+sll+sfyv+p lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 67 ATGKLVGCSAIVASAGYSEPFYSFRNETFVHASRELKIHNKIHVLSQCHDLTGNSLLTSFYVKP 130 **************************************************************** PP TIGR03245 129 elreaeaaellsrarllfiaaererfasrviaellGvsdeaGesPfWdaiGrkffdldfaaver 192 el+++ +ael+sr+rllf+a+++erfa++v++e++G sde+G+sPfWdaiGr+ffdl++aa+er lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 131 ELVGSPWAELNSRGRLLFVASHPERFADSVVTEIVGYSDENGDSPFWDAIGRNFFDLNYAAAER 194 **************************************************************** PP TIGR03245 193 asglksktfiaelmPsyPiyvpllpdeaqealgqvheraelafdlllreGfeadryidifdgGp 256 ++glks+tf+aelmP+yPiyvpllpd+aqea+gqvh+ra+++fd+l+reGfe+d+yidifdgGp lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 195 LCGLKSRTFLAELMPHYPIYVPLLPDSAQEAMGQVHPRAQITFDILMREGFETDHYIDIFDGGP 258 **************************************************************** PP TIGR03245 257 vleaeldrlktvkksrekpvkieeaatgg.stylvsnglledfravladlavvdGkalrlkada 319 +l+a++++++++++sr++pvki e +g ++ylv+n +l+d+ravl++l+ ++Gk+++l+ +a lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 259 TLHARVSGIRSIAQSRVVPVKIGEPVKGAgRQYLVANAQLQDYRAVLLELDYAPGKPVTLDLEA 322 ************************999888********************************** PP TIGR03245 320 aealgvseGdavrlval 336 aealgv+eG++vrlva+ lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3442 323 AEALGVGEGASVRLVAV 339 **************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (336 nodes) Target sequences: 1 (339 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.23 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory