Align Arginine N-succinyltransferase; AST; AOST; EC 2.3.1.109 (characterized)
to candidate Pf1N1B4_5857 Arginine N-succinyltransferase (EC 2.3.1.109)
Query= SwissProt::P0AE37 (344 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 Length = 344 Score = 295 bits (756), Expect = 9e-85 Identities = 153/343 (44%), Positives = 214/343 (62%), Gaps = 2/343 (0%) Query: 2 MVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAIKTWQGELPKSEQGYVFV 61 M+IRPV+ +D+ AL+ L + G G T+LPA+E L+ R+ A +T+ ++ +++ Y+FV Sbjct: 1 MIIRPVQVTDLPALLDLVQRAGPGFTTLPASEERLTHRVRWAQRTFAEQVERADADYLFV 60 Query: 62 LEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELNVYNALPTLFLSNDHTGSSE 121 LED + V G+ A+ AVGL +PWYNYRVG V +S +L + LPTLFL+N+ TG SE Sbjct: 61 LEDDDQ-QVVGVSALVGAVGLREPWYNYRVGLTVSSSPDLGIARQLPTLFLNNEMTGQSE 119 Query: 122 LCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVVAEMRGVIDEHGYSPFWQSLGK 181 +C+LFL D R NG LLS R +F+A F F DK++AE+RG DE G SPFW SLG+ Sbjct: 120 ICSLFLRHDQRHGNNGRLLSLGRLLFVAEFPHLFGDKLIAELRGSADEQGCSPFWDSLGR 179 Query: 182 RFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLSQEAQDVIGQVHPQTAPARAVLEK 241 FF MDFS AD L G G KAFIAELMP+ P+YT L+++AQ VIG+ HP T PA +L Sbjct: 180 HFFKMDFSHADHLSGLGSKAFIAELMPRQPLYTCLLTEQAQAVIGKPHPNTEPALKILTA 239 Query: 242 EGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVEVAEGQPAQGDFPACLVANENYHHFR 301 EGF ++ YIDIFD GP +E + ++R +R S+ +E+A G P L+ N + R Sbjct: 240 EGFSHKGYIDIFDAGPVIEAPVSKIRTVRDSQRLELAIG-TLDDQAPIWLIHNRRLENCR 298 Query: 302 VVLVRTDPATERLILTAAQLDALKCHAGDRVRLVRLCAEEKTA 344 + + LI+ L+ G+ VR V L ++ A Sbjct: 299 ITTAQARLVGNSLIVDRLTAKRLQLQPGNSVRAVPLRNRQQQA 341 Lambda K H 0.321 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 330 Number of extensions: 15 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 344 Length of database: 344 Length adjustment: 29 Effective length of query: 315 Effective length of database: 315 Effective search space: 99225 Effective search space used: 99225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 49 (23.5 bits)
Align candidate Pf1N1B4_5857 (Arginine N-succinyltransferase (EC 2.3.1.109))
to HMM TIGR03244 (astA: arginine N-succinyltransferase (EC 2.3.1.109))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03244.hmm # target sequence database: /tmp/gapView.17566.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03244 [M=336] Accession: TIGR03244 Description: arg_catab_AstA: arginine N-succinyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.9e-138 446.9 0.0 2.2e-138 446.7 0.0 1.0 1 lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 Arginine N-succinyltransferase ( Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 Arginine N-succinyltransferase (EC 2.3.1.109) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 446.7 0.0 2.2e-138 2.2e-138 1 336 [] 2 334 .. 2 334 .. 0.99 Alignments for each domain: == domain 1 score: 446.7 bits; conditional E-value: 2.2e-138 TIGR03244 1 ivrpvktsdldallelakeaGvGltslpaneellekrieraeksfageleraeegylfvledte 64 i+rpv+ +dl+all+l ++aG G+t+lpa ee+l++r+ +a+++fa+++era+++ylfvled + lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 2 IIRPVQVTDLPALLDLVQRAGPGFTTLPASEERLTHRVRWAQRTFAEQVERADADYLFVLED-D 64 79************************************************************.8 PP TIGR03244 65 tgkvvGvsaieaavGleepfynyrvgkvvhaskelniykkletlflsndltgaselCtlfldee 128 +++vvGvsa+ avGl+ep+ynyrvg +v++s +l+i ++l+tlfl n++tg+se+C+lfl+++ lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 65 DQQVVGVSALVGAVGLREPWYNYRVGLTVSSSPDLGIARQLPTLFLNNEMTGQSEICSLFLRHD 128 999************************************************************* PP TIGR03244 129 yrkelnGkllskarflflaefkerfskkiiaemrGvsdeeGrsPfWealgkkffsldfskadyl 192 r+++nG+lls r+lf+aef+++f++k+iae+rG +de+G sPfW++lg++ff++dfs+ad+l lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 129 QRHGNNGRLLSLGRLLFVAEFPHLFGDKLIAELRGSADEQGCSPFWDSLGRHFFKMDFSHADHL 192 **************************************************************** PP TIGR03244 193 sgiGkkafiaelmPkfPiyvdllskeaqdvigkvhektkPalalleseGlryqgyvdifdaGpt 256 sg+G+kafiaelmP+ P+y++ll+++aq+vigk h++t+Pal++l +eG+ ++gy+difdaGp+ lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 193 SGLGSKAFIAELMPRQPLYTCLLTEQAQAVIGKPHPNTEPALKILTAEGFSHKGYIDIFDAGPV 256 **************************************************************** PP TIGR03244 257 leaevakiravresklvevavaesaaedeaepylvanekledfrvvlvessldaeelvlsaeea 320 +ea v+kir+vr+s+++e+a+++ + +a+ l++n++le++r+++++++l ++l++++ +a lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 257 IEAPVSKIRTVRDSQRLELAIGTLDD--QAPIWLIHNRRLENCRITTAQARLVGNSLIVDRLTA 318 *********************97654..5699******************************** PP TIGR03244 321 kalkveeGdkvrvval 336 k+l+++ G++vr v+l lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5857 319 KRLQLQPGNSVRAVPL 334 *************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (336 nodes) Target sequences: 1 (344 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 8.14 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory