Align putrescine-pyruvate transaminase (EC 2.6.1.113) (characterized)
to candidate Pf1N1B4_2257 Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18)
Query= BRENDA::Q9I6J2 (456 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2257 Length = 400 Score = 647 bits (1668), Expect = 0.0 Identities = 297/371 (80%), Positives = 339/371 (91%) Query: 7 NAKTREWQALSRDHHLPPFTDYKQLNEKGARIITKAEGVYIWDSEGNKILDAMAGLWCVN 66 N +TREWQALS DHHL PF+D+KQL EKG RIIT A+GVY+WDSEGNKILD MAGLWCV Sbjct: 5 NPQTREWQALSSDHHLAPFSDFKQLKEKGPRIITSAKGVYLWDSEGNKILDGMAGLWCVA 64 Query: 67 VGYGREELVQAATRQMRELPFYNLFFQTAHPPVVELAKAIADVAPEGMNHVFFTGSGSEA 126 +GYGR+EL AA++QMRELP+YNLFFQTAHPPV+ELAK I+DVAPEGMNHVFFTGSGSE Sbjct: 65 IGYGRDELADAASKQMRELPYYNLFFQTAHPPVLELAKVISDVAPEGMNHVFFTGSGSEG 124 Query: 127 NDTVLRMVRHYWATKGQPQKKVVIGRWNGYHGSTVAGVSLGGMKALHEQGDFPIPGIVHI 186 NDT+LRMVRHYWA KGQP+KKV+I R NGYHGSTVAG SLGGM +HEQGD PIPGIVHI Sbjct: 125 NDTMLRMVRHYWAIKGQPKKKVIISRKNGYHGSTVAGASLGGMTYMHEQGDLPIPGIVHI 184 Query: 187 AQPYWYGEGGDMSPDEFGVWAAEQLEKKILEVGEENVAAFIAEPIQGAGGVIVPPDTYWP 246 AQPYW+GEGGDM+P+EFG+WAA QLE+KILEVG +NV AFIAEPIQGAGGVI+PPD+YWP Sbjct: 185 AQPYWFGEGGDMTPNEFGIWAANQLEEKILEVGVDNVGAFIAEPIQGAGGVIIPPDSYWP 244 Query: 247 KIREILAKYDILFIADEVICGFGRTGEWFGSQYYGNAPDLMPIAKGLTSGYIPMGGVVVR 306 +I+EILAKYDILF+ADEVICGFGRTGEWFG+ +Y P +M IAKGLTSGYIPMGG++VR Sbjct: 245 RIKEILAKYDILFVADEVICGFGRTGEWFGTDFYDLKPHMMTIAKGLTSGYIPMGGLIVR 304 Query: 307 DEIVEVLNQGGEFYHGFTYSGHPVAAAVALENIRILREEKIIEKVKAETAPYLQKRWQEL 366 D++VEVLN+GG+F HGFTYSGHPVAAAVALENIRILR+EKI+E+V+ ETAPYLQKR +EL Sbjct: 305 DDVVEVLNEGGDFNHGFTYSGHPVAAAVALENIRILRDEKIVERVRTETAPYLQKRLREL 364 Query: 367 ADHPLVGEARG 377 +DHPLVGE RG Sbjct: 365 SDHPLVGEVRG 375 Lambda K H 0.320 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 712 Number of extensions: 29 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 456 Length of database: 400 Length adjustment: 32 Effective length of query: 424 Effective length of database: 368 Effective search space: 156032 Effective search space used: 156032 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory