Alignments for a candidate for TM0027 in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for TM0027 in Pseudomonas fluorescens FW300-N1B4

Align TM0027, component of β-glucoside porter (Conners et al., 2005). Binds cellobiose, laminaribiose (Nanavati et al. 2006). Regulated by cellobiose-responsive repressor BglR (characterized)
to candidate Pf1N1B4_5629 Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)

Query= TCDB::Q9WXN4
         (268 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5629
          Length = 524

 Score =  160 bits (406), Expect = 4e-44
 Identities = 96/257 (37%), Positives = 154/257 (59%), Gaps = 9/257 (3%)

Query: 4   LVVKNLTKIFSLG---FFSKRRIEAVKNVSFEVKEKEIVSLVGESGSGKTTTAKMILRLL 60
           L V NL   F++G   F  K  + AV  +S  V+  + + +VGESGSGK+T  + ILRLL
Sbjct: 270 LEVDNLQVHFAIGGGLFQRKTYLRAVDGISLNVQRGKTLGIVGESGSGKSTLGQAILRLL 329

Query: 61  PPTSGEIYFEGKDIWKDIKDRESLVEFRRKVHAVFQDPFASYNPFYPVERTLWQAISLLE 120
             + G I F+G+ +  D   ++ L  +R+K+  VFQDPF S +P   V + + + + +  
Sbjct: 330 D-SEGSIRFQGEAL--DGLTQKQLRPWRKKMQVVFQDPFGSLSPRMSVAQIISEGLEVHS 386

Query: 121 NKPSNKKEALELIKESLFRVGIDPKDVLGKYPHQISGGQKQRIMIARCWILRPLLIVADE 180
               ++ EA  +    L  VG+DP+    +YPH+ SGGQ+QRI IAR  +L+P LI+ DE
Sbjct: 387 QSTPDECEAQVI--RVLEEVGLDPQS-RHRYPHEFSGGQRQRIAIARALVLKPALILLDE 443

Query: 181 PTSMIDASSRGGIIKLLEELREEQGTSIIFITHDLGLAYYVSDNIFVMKNGEIVERGHPD 240
           PTS +D + +  ++ LL +L+E+ G + +FI+HDL +   ++ ++ V+K+G++VE G   
Sbjct: 444 PTSALDRTVQKQVVALLRQLQEKYGLTYLFISHDLAVIRALAHDMIVIKDGKVVESGASH 503

Query: 241 KVVLEPTHEYTKLLVGS 257
            V   P H YTK L+ +
Sbjct: 504 DVFDSPQHPYTKELLAA 520



 Score =  156 bits (395), Expect = 8e-43
 Identities = 95/242 (39%), Positives = 149/242 (61%), Gaps = 12/242 (4%)

Query: 26  VKNVSFEVKEKEIVSLVGESGSGKTTTAKMILRLLPPT----SGEIYFEGKD-IWKDIKD 80
           V+N+  +++  E ++LVGESG GK+ TA  IL+LLP T    +G I + G++ I  DIK 
Sbjct: 21  VRNLCLDIRPGECLALVGESGCGKSVTAHSILQLLPETGTETTGSIRYRGQELIGTDIK- 79

Query: 81  RESLVEFR-RKVHAVFQDPFASYNPFYPVERTLWQAISLLENKPSNKKEALELIKESLFR 139
             +L E R  ++  +FQ+P  S NP + VE+ + +  +LL ++    K A   I E L  
Sbjct: 80  --TLRELRGNRIAMIFQEPMTSLNPLHSVEKQIGE--TLLLHRGLGGKAAQARILELLQL 135

Query: 140 VGID-PKDVLGKYPHQISGGQKQRIMIARCWILRPLLIVADEPTSMIDASSRGGIIKLLE 198
           VGI  P++ L  YPHQ+SGGQ+QR+MIA      P L++ADEPT+ +D + +  I+ LL+
Sbjct: 136 VGIQKPEERLKAYPHQLSGGQRQRVMIAMALACEPELLIADEPTTALDVTVQRKILLLLK 195

Query: 199 ELREEQGTSIIFITHDLGLAYYVSDNIFVMKNGEIVERGHPDKVVLEPTHEYTKLLVGSI 258
            L++  G S++ I+HDL L   ++  + VMK GEIVE+   + +  EP H Y+ +L+ + 
Sbjct: 196 SLQQRLGMSLLLISHDLNLVRSIAQRVCVMKAGEIVEQAPCETLFTEPKHPYSCVLLHAE 255

Query: 259 PK 260
           P+
Sbjct: 256 PE 257


Lambda     K      H
   0.319    0.139    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 371
Number of extensions: 18
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 268
Length of database: 524
Length adjustment: 30
Effective length of query: 238
Effective length of database: 494
Effective search space:   117572
Effective search space used:   117572
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources