Align ABC transporter substrate-binding protein; SubName: Full=Histidine transport system substrate-binding protein (characterized, see rationale)
to candidate Pf1N1B4_4791 Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1)
Query= uniprot:A0A1N7UK26 (258 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4791 Length = 259 Score = 295 bits (755), Expect = 6e-85 Identities = 139/249 (55%), Positives = 191/249 (76%), Gaps = 1/249 (0%) Query: 10 ALLLPLCAT-AHAQEWKEIRFGVFPEYPPFESVAADGSLQGFDIELGNAICAKLEVKCTW 68 AL L C+T A A+EWKE+RFGV P YPPFES ADG +QGF ++LGNAICA+L++KC W Sbjct: 10 ALALLCCSTGASAKEWKELRFGVNPSYPPFESTTADGGVQGFGVDLGNAICAELKLKCVW 69 Query: 69 VHNEFDGMIPALRARKFDAIMSSMAVTPAREKIIDFSDRLFLSPTSVITRKSADFGDTPE 128 V N+FDG+IPAL+A KFDAI SSM VT AR+K IDF+DRL+ PT+++TRK + T E Sbjct: 70 VSNDFDGLIPALKAGKFDAIESSMTVTDARKKQIDFTDRLYAGPTAIVTRKDSGLLPTAE 129 Query: 129 SLMGKQVGVLQGSLQEAYARAHLAKLGAQIKAYQSQDQNYADLQNGRLDATLTDKLEAQL 188 SL GK +G +QG++QE YA+A L G +++AYQ+QDQ YADL GRLDA++ DKL+AQ+ Sbjct: 130 SLRGKTIGYMQGTIQETYAKAKLGPGGVKLRAYQNQDQVYADLVFGRLDASIQDKLQAQM 189 Query: 189 NFLSKPEGSDFKTGPAFKDPTLPLDIAMGLRKNDQALRALINKGIAAVQADGTYAQIQKK 248 +FL+ P+G+DF+ DP +P +IA+G+RK+++ L+ ++N I A+ G YAQIQ+K Sbjct: 190 SFLTSPQGADFQNSEGISDPLVPSEIAIGIRKDNEELKGMLNAAIKALHEKGIYAQIQQK 249 Query: 249 YFGDQDIYH 257 +FGD D+Y+ Sbjct: 250 HFGDLDLYN 258 Lambda K H 0.319 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 228 Number of extensions: 9 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 258 Length of database: 259 Length adjustment: 24 Effective length of query: 234 Effective length of database: 235 Effective search space: 54990 Effective search space used: 54990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory