Alignments for a candidate for arcB in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for arcB in Pseudomonas fluorescens FW300-N1B4

Align Ornithine carbamoyltransferase 1, anabolic; OTCase 1; Ornithine carbamoyltransferase 1, phaseolotoxin-sensitive; SOCT; EC 2.1.3.3 (characterized)
to candidate Pf1N1B4_762 Ornithine carbamoyltransferase (EC 2.1.3.3)

Query= SwissProt::Q02047
         (306 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_762
          Length = 307

 Score =  537 bits (1383), Expect = e-157
 Identities = 268/306 (87%), Positives = 284/306 (92%)

Query: 1   MNARHFLSMMDYTPDELLGLIRRGVELKDLRIRGELFEPLKNRVLGMIFEKSSTRTRLSF 60
           M+ARHFLS+MD TP+EL+ +IRRGVELKDLR RG LFEPLKNRVLGMIFEKSSTRTR+SF
Sbjct: 2   MSARHFLSLMDCTPEELVSVIRRGVELKDLRNRGVLFEPLKNRVLGMIFEKSSTRTRISF 61

Query: 61  EAGMIQLGGQAIFLSHRDTQLGRGEPIADSAKVMSRMLDAVMIRTYAHSNLTEFAANSRV 120
           EAGMIQLGGQAIFLS RDTQLGRGEPI D A VMS MLDAVMIRT+AHS LTEFAANSRV
Sbjct: 62  EAGMIQLGGQAIFLSPRDTQLGRGEPIGDCAIVMSSMLDAVMIRTFAHSTLTEFAANSRV 121

Query: 121 PVINGLSDDLHPCQLLADMQTFLEHRGSIKGKTVAWIGDGNNMCNSYIEAAIQFDFQLRV 180
           PVINGLSDDLHPCQLLADMQTFLEHRGSI+GKTVAWIGDGNNMCNSYIEAAIQFDFQLR+
Sbjct: 122 PVINGLSDDLHPCQLLADMQTFLEHRGSIQGKTVAWIGDGNNMCNSYIEAAIQFDFQLRI 181

Query: 181 ACPAGYEPNPEFLALAGERVTIVRDPKAAVAGAHLVSTDVWTSMGQEEETARRMALFAPF 240
           ACP GYEPNPEF+A AG+RVTIVRDP+ AV GAHLVSTDVWTSMGQEEETA+R+ LFAPF
Sbjct: 182 ACPEGYEPNPEFVAKAGDRVTIVRDPQEAVRGAHLVSTDVWTSMGQEEETAKRLKLFAPF 241

Query: 241 QVTRASLDLAEKDVLFMHCLPAHRGEEISVDLLDDSRSVAWDQAENRLHAQKALLEFLVA 300
           QV RA LDLA  DVLFMHCLPAHRGEEIS+DLLDDSRSVAWDQAENRLHAQKALLEFLV 
Sbjct: 242 QVNRALLDLAAPDVLFMHCLPAHRGEEISLDLLDDSRSVAWDQAENRLHAQKALLEFLVP 301

Query: 301 PSHQRA 306
           PS+  A
Sbjct: 302 PSYHHA 307


Lambda     K      H
   0.322    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 373
Number of extensions: 4
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 306
Length of database: 307
Length adjustment: 27
Effective length of query: 279
Effective length of database: 280
Effective search space:    78120
Effective search space used:    78120
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate Pf1N1B4_762 (Ornithine carbamoyltransferase (EC 2.1.3.3))
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00658.hmm
# target sequence database:        /tmp/gapView.22803.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00658  [M=304]
Accession:   TIGR00658
Description: orni_carb_tr: ornithine carbamoyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                     Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                     -----------
   2.7e-118  380.6   0.0     3e-118  380.4   0.0    1.0  1  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_762  Ornithine carbamoyltransferase (


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_762  Ornithine carbamoyltransferase (EC 2.1.3.3)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  380.4   0.0    3e-118    3e-118       1     303 [.       5     300 ..       5     301 .. 0.99

  Alignments for each domain:
  == domain 1  score: 380.4 bits;  conditional E-value: 3e-118
                                     TIGR00658   1 rhllslldlseeelkellelakklkkekkkgkeekklkgktlaliFekrstRtRvsfevaayelG 65 
                                                   rh+lsl+d ++eel++++ ++ +lk  +++g+  + lk++ l++iFek+stRtR+sfe+++++lG
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_762   5 RHFLSLMDCTPEELVSVIRRGVELKDLRNRGVLFEPLKNRVLGMIFEKSSTRTRISFEAGMIQLG 69 
                                                   8**************************************************************** PP

                                     TIGR00658  66 aqvlylnkeelqlgrkesikDtarvlsryvdaivvRvykhedveelakyasvPvingLtdlehPc 130
                                                   +q+++l++ ++qlgr+e+i D a v+s+++da+++R+++h++++e+a ++ vPvingL+d  hPc
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_762  70 GQAIFLSPRDTQLGRGEPIGDCAIVMSSMLDAVMIRTFAHSTLTEFAANSRVPVINGLSDDLHPC 134
                                                   ***************************************************************** PP

                                     TIGR00658 131 qilaDlltikeklgklkevklvyvGDannvanslllaaaklGldvvvatPeglepeaeivkkakk 195
                                                   q+laD++t  e+ g++++ +++++GD+nn++ns + aa ++ +++++a+Peg+ep++e v ka  
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_762 135 QLLADMQTFLEHRGSIQGKTVAWIGDGNNMCNSYIEAAIQFDFQLRIACPEGYEPNPEFVAKA-- 197
                                                   **********************************************************99988.. PP

                                     TIGR00658 196 iakenggkleltedpkkavkdadviytDvwvsmGeeekkeerlkllkpyqvneellelakpevkf 260
                                                        g ++++++dp++av++a+++ tDvw+smG+ee++++rlkl+ p+qvn+ ll+la p+v f
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_762 198 -----GDRVTIVRDPQEAVRGAHLVSTDVWTSMGQEEETAKRLKLFAPFQVNRALLDLAAPDVLF 257
                                                   .....88********************************************************** PP

                                     TIGR00658 261 lhCLPavrGeevtdevlegeasivfdeaenRlhaqkavlkall 303
                                                   +hCLPa+rGee++ + l+ ++s+ +d+aenRlhaqka+l++l+
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_762 258 MHCLPAHRGEEISLDLLDDSRSVAWDQAENRLHAQKALLEFLV 300
                                                   ***************************************9975 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (307 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 10.48
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources