GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gcdH in Pseudomonas fluorescens FW300-N1B4

Align glutaryl-CoA dehydrogenase (ETF) (EC 1.3.8.6) (characterized)
to candidate Pf1N1B4_4789 Butyryl-CoA dehydrogenase (EC 1.3.99.2)

Query= BRENDA::Q3JP94
         (395 letters)



>lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4789 Butyryl-CoA
           dehydrogenase (EC 1.3.99.2)
          Length = 383

 Score =  211 bits (537), Expect = 3e-59
 Identities = 122/374 (32%), Positives = 202/374 (54%), Gaps = 2/374 (0%)

Query: 15  DQQLADDERMVRDAAHAYAQGKLAPRVTEAFRHETTDAAIFREMGEIGLLGPTIPEQYGG 74
           D +L++++ M+RD A  +A+G++AP      +    D  +  +MGE+GLLG  +PE++GG
Sbjct: 3   DIELSEEQVMIRDMARDFARGEIAPHAQAWEKAGWIDDGLVAKMGELGLLGMVVPEEWGG 62

Query: 75  PGLDYVSYGLIAREVERVDSGYRSMMSVQSSLVMVPIFEFGSDAQKEKYLPKLATGEWIG 134
             +DYV+Y L   E+   D    ++MS+ +S+   P+  +G++ QK+++LP LA+G+ IG
Sbjct: 63  TYVDYVAYALAVEEISAGDGATGALMSIHNSVGCGPVLNYGTEEQKQQWLPDLASGQAIG 122

Query: 135 CFGLTEPNHGSDPGSMVTRARKVPGGYSLSGSKMWITNSPIADVFVVWAKLD-EDGRDEI 193
           CF LTEP  GS+  ++ TRA    G + ++G+K +++N   A + +V+A  D E G+  I
Sbjct: 123 CFCLTEPQAGSEAHNLRTRAELRDGQWVINGAKQFVSNGKRAKLAIVFAVTDPELGKRGI 182

Query: 194 RGFILEKGCKGLSAPAIHGKVGLRASITGEIVLDEAFVPEENIL-PHVKGLRGPFTCLNS 252
             F++     G        K+G+RAS T  + L+   VPE N+L    KGL    + L  
Sbjct: 183 SAFLVPTETAGFIVDRSEHKMGIRASDTCAVTLNNCTVPEANLLGERGKGLAIALSNLEG 242

Query: 253 ARYGIAWGALGAAESCWHIARQYVLDRKQFGRPLAANQLIQKKLADMQTEITLGLQGVLR 312
            R GIA  ALG A + +  A  Y  DR QF +P+  +Q I   LADM T +      +L 
Sbjct: 243 GRIGIAAQALGIARAAFEAALAYARDRVQFDKPIIEHQSIANMLADMHTRLNAARLLILH 302

Query: 313 LGRMKDEGTAAVEITSIMKRNSCGKALDIARLARDMLGGNGISDEFGVARHLVNLEVVNT 372
             R++  G   +   S  K  +   A  +   A  + GG G  +++ V R+  +  +   
Sbjct: 303 AARLRSAGKPCLSEASQAKLFASEMAEKVCSSAIQIHGGYGYLEDYPVERYYRDARITQI 362

Query: 373 YEGTHDIHALILGR 386
           YEG+ +I  +++ R
Sbjct: 363 YEGSSEIQRMVIAR 376


Lambda     K      H
   0.320    0.138    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 372
Number of extensions: 18
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 395
Length of database: 383
Length adjustment: 30
Effective length of query: 365
Effective length of database: 353
Effective search space:   128845
Effective search space used:   128845
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory