Align 2-deoxy-D-ribonate transporter 1 (characterized)
to candidate Pf1N1B4_70 Major facilitator family transporter
Query= reanno::WCS417:GFF1429 (438 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_70 Length = 441 Score = 328 bits (842), Expect = 2e-94 Identities = 166/411 (40%), Positives = 246/411 (59%), Gaps = 8/411 (1%) Query: 21 LMPLLIIAYILSFLDRTNIALAKHHLDVDLGISAAAYGLGAGLFFLTYALSEIPSNLIMH 80 ++PL +I +I++++DR NI + H++ DLGI AAAYG GAGLFF+ YAL E+PSN+++ Sbjct: 20 VLPLFVIMFIVNYIDRVNIGFVRAHMEHDLGIGAAAYGFGAGLFFIGYALFEVPSNILLQ 79 Query: 81 KVGARFWIARIMVTWGLISAAMAFVQGETSFYVLRLLLGIAEAGLFPGVMLYLTYWFNRE 140 KVGAR W+ RIM+TWGL++A MAF+Q ET FY+LR LLG+AEAG FPGV+ Y T W Sbjct: 80 KVGARIWLTRIMLTWGLVAACMAFIQNETHFYILRFLLGVAEAGFFPGVIYYFTRWLPGV 139 Query: 141 QRARATGYFLLGVCFANIIGGPVGAALMRMDGMLGWHGWQWMFMLEGLPAVAFAWVVWRK 200 +R +A FL G A++I GP+ L+++ G LG HGWQWM+ +EG+ +V VW Sbjct: 140 ERGKAIAIFLSGSAIASLISGPLSGLLLQISG-LGMHGWQWMYFIEGMFSVGLCVFVWFW 198 Query: 201 LPDRPSKAPWLSAEEARGIEQRIAQETEEGAGEGGHSLKNWL-----TPQILLAIFVYFC 255 L +P A WL+ E + + I + E+ A E +K L QI+L +YF Sbjct: 199 LDSKPHDAKWLTLPEQDALVKAI--DDEQLAREAATPIKPSLGTLLKDRQIILFCVIYFF 256 Query: 256 HQITIYTVIFFLPSIISKYGELSTMSVGLLTSLPWIAAALGALLIPRFATTPGRCRRLLV 315 Q+TIY F+LPSII K G+LS + VGL S+PW+ + +G + + + Sbjct: 257 IQLTIYAATFWLPSIIKKMGDLSDIQVGLFNSIPWLLSIVGMYAFATLSAKWKHQQAWVA 316 Query: 316 TGLLTMALGLGIASVSGPVFSLLGFCLSAVMFFVVQSIIFLYPASRLKGVALAGGLGFVN 375 LL A G+ +++ GP+F+ + C +A+ F S+ + P + L AG + +N Sbjct: 317 AALLIAAAGMFMSTTGGPIFAFVAICFAALGFKSASSLFWPIPQAYLDARIAAGVIALIN 376 Query: 376 ACGLLGGFVGPSVMGVIEQSTGNAMNGLKVIALVLVVAALAALRLRMGHEP 426 + G LGGFV P+ G++EQ TG+ GL +A ++AA+ R +P Sbjct: 377 SVGNLGGFVAPTTFGLLEQHTGSIQGGLYGLAATSIIAAIIVFAARNKPKP 427 Lambda K H 0.327 0.141 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 574 Number of extensions: 34 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 438 Length of database: 441 Length adjustment: 32 Effective length of query: 406 Effective length of database: 409 Effective search space: 166054 Effective search space used: 166054 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory