Alignments for a candidate for drdehyd-beta in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for drdehyd-beta in Pseudomonas fluorescens FW300-N1B4

Align 2-deoxy-D-ribose dehydrogenase β subunit (characterized)
to candidate Pf1N1B4_4869 Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)

Query= metacyc::MONOMER-20833
         (739 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4869
          Length = 746

 Score =  347 bits (889), Expect = 2e-99
 Identities = 261/747 (34%), Positives = 379/747 (50%), Gaps = 39/747 (5%)

Query: 3   LINELLDAPVNLSRRRFLASTAVGALVIGFGLPLGAGRVQAATSA---ERGTQVPAFLEI 59
           L  + +D P  L RR FL    VG L +G    L   +      A   +   Q  AF++I
Sbjct: 18  LERDQIDGPTTLPRRSFLKILGVGGLALGAFPHLALAQQPGDNRAMPLKPSQQPSAFVQI 77

Query: 60  RPDGTVRLLSPFMEGGQGTHTAMAQIVGEELDADPATFIVEAAPPGEAYVVMENGLRITG 119
            P+G V +    +E GQG  T +  I+ EELDAD +           AY     G+ +TG
Sbjct: 78  APNGEVTVTINRLEFGQGVQTGLPMILAEELDADWSLVRSRNGTSDIAYQDPLFGMHLTG 137

Query: 120 GSMSVRMSYPTMRRLGALARAMLLQAGAKQWGVPVGELTTQPGRVVHAASGRSLGYGELA 179
           GS S++ SY   R LGA ARAMLL A A +W V +  L+TQ G V+  A GR   YGELA
Sbjct: 138 GSNSIKNSYTQYRELGARARAMLLAAAAARWNVDMASLSTQSGMVLGPA-GRKASYGELA 196

Query: 180 SSALDMPVPDPASITLRDPSQFRWIGKPVKRLDAYDKSTGKALYSIDLKVDNMLHAAVQH 239
            +A+ +PVP+  +ITL+DP  FR IG    R+DA  KS+G+  + ID+K+   L A V  
Sbjct: 197 EAAMALPVPE--TITLKDPKDFRIIGHATTRIDAKAKSSGQQDFGIDMKLPGQLTAVVAR 254

Query: 240 APRLGMTVGSLRNQSQVEGMKGVHSVHVLP---GA--VAVVAERWWHAKRAVEAIQVDWQ 294
            P  G  + SL + S    +KGV +V  +P   GA  VAVVA+ +W AK+  +A+++ W 
Sbjct: 255 PPVFGAKIASL-DDSAARAIKGVKAVLRVPLDGGAEGVAVVADGYWQAKQGRDALKLQWD 313

Query: 295 EAAADSALRVMPADFSSDKHREFLAAQQGPTRDDENEGDVAGALKGAKTQVEATYHNQYL 354
               +     + +D     +RE LA + GP      E D+A  L+ A  +++A +   YL
Sbjct: 314 TTTVEK----VDSDKQLADYRE-LAGRPGPRL---FEADMA-PLETAPHRLDAEFVFPYL 364

Query: 355 NHAQLEPPSALARFNPDGTLEIWLPNQAPDMFRADIAKRTGLDPAQITLHSPLLGGFFGR 414
            HA +EP +   + + +   E+W+  Q      A  A+   L P QI ++    GG FGR
Sbjct: 365 AHAPMEPLNCTVKLSEE-RCELWVGTQFAGADSAAAARVLNLKPEQIQINVQTAGGGFGR 423

Query: 415 HFLYDSANPYPQAIALAKA-----VSRPIKLIWSREEEFLRDVLRPLAVVKFRAALDDKG 469
             +  S +    A  +AKA     ++ P++ +WSRE++      RP+ + + R   DD G
Sbjct: 424 RGIATS-DIVVLACEVAKAARMAGLNVPVRTLWSREDDIKGGYYRPMHLHRARLGFDDTG 482

Query: 470 LPVAIEAVSATEGPTEA--LAGKQGDKIDPTAVEGLSGKSYAIPNKRIAQIYVKGAPMLG 527
             +A + V   +         G+  + IDPTA EG+    Y +P +          P+L 
Sbjct: 483 KVLAWDHVVVGQSIISGTMFGGRVKNGIDPTATEGMR-DPYPLPMRLTVHHPKLNVPVL- 540

Query: 528 YWRSVGNSLNDFFYESFLDELADKGGHDPYELRLHLLRD-NPRLTTLLQAAGELSGGWKR 586
           +WRSVG++   F  E+ +DE+A     DP   R+ L  D +PR    LQ A + S   KR
Sbjct: 541 WWRSVGSTHTAFVMETVIDEIARTTKQDPVAYRMKLFADQSPRHRAALQLAVDKSEYGKR 600

Query: 587 GPFTAEDGTRRARGVAMASPFGSHTAAIAEVSIENGKVKVHDIWQAIDPGSIVNPAIVEA 646
                E    RA GVA+   F S  A + E S++NG+  +H +   +    +VNP  VEA
Sbjct: 601 -----ELPAGRAWGVAVHESFSSVVAYVVEASVQNGRPVLHHVTAGVHCNLVVNPRSVEA 655

Query: 647 QVNGAVALGLSQTLLEEAVYV-DGKPRARNYDLYPILAPAQMARVHVRVVESGEKMGGIG 705
           QV GA  +GLS  L   A+ + DG  +  N+  + +     M    + VV S E   G+G
Sbjct: 656 QVQGAALMGLSMCLPGGAITLKDGVVQQSNFADFSVPRITDMPEFSIHVVPSAEPPTGMG 715

Query: 706 EPPLPAVAPAVANAVAQLTGQRVRSLP 732
           EP LP +APA ANAVA+LTG+ VR LP
Sbjct: 716 EPGLPPLAPAFANAVARLTGKPVRELP 742


Lambda     K      H
   0.317    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1408
Number of extensions: 75
Number of successful extensions: 13
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 739
Length of database: 746
Length adjustment: 40
Effective length of query: 699
Effective length of database: 706
Effective search space:   493494
Effective search space used:   493494
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources