GapMind for catabolism of small carbon sources

 

L-fucose catabolism in Pseudomonas fluorescens FW300-N1B4

Best path

HSERO_RS05250, HSERO_RS05255, HSERO_RS05260, fucU, fucI, fucK, fucA, tpi, aldA

Also see fitness data for the top candidates

Rules

Overview: Fucose degradation in GapMind is based on the MetaCyc pathway via L-fuculose (link) or the oxidative pathway via 2,4-diketo-3-deoxy-L-fuconate (KDF) hydrolase (PMC6336799).

23 steps (14 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
HSERO_RS05250 ABC transporter for L-fucose, ATPase component Pf1N1B4_6034 Pf1N1B4_4286
HSERO_RS05255 ABC transporter for L-fucose, permease component Pf1N1B4_6033 Pf1N1B4_4287
HSERO_RS05260 ABC transporter for L-fucose, substrate-binding component
fucU L-fucose mutarotase FucU
fucI L-fucose isomerase FucI
fucK L-fuculose kinase FucK
fucA L-fuculose-phosphate aldolase FucA Pf1N1B4_5075
tpi triose-phosphate isomerase Pf1N1B4_1164 Pf1N1B4_2399
aldA lactaldehyde dehydrogenase Pf1N1B4_5695 Pf1N1B4_171
Alternative steps:
BPHYT_RS34240 ABC transporter for L-fucose, permease component Pf1N1B4_409
BPHYT_RS34245 ABC transporter for L-fucose, ATPase component Pf1N1B4_4286 Pf1N1B4_6034
BPHYT_RS34250 ABC transporter for L-fucose, substrate-binding component
fdh L-fucose 1-dehydrogenase Pf1N1B4_512 Pf1N1B4_4954
fucD L-fuconate dehydratase Pf1N1B4_1107
fucDH 2-keto-3-deoxy-L-fuconate 4-dehydrogenase Pf1N1B4_512 Pf1N1B4_412
fucO L-lactaldehyde reductase Pf1N1B4_3270 Pf1N1B4_2542
fuconolactonase L-fucono-1,5-lactonase
fucP L-fucose:H+ symporter FucP
KDF-hydrolase 2,4-diketo-3-deoxy-L-fuconate hydrolase Pf1N1B4_5706 Pf1N1B4_2275
SM_b21103 ABC transporter for L-fucose, substrate-binding component
SM_b21104 ABC transporter for L-fucose, permease component 1
SM_b21105 ABC transporter for L-fucose, permease component 2 Pf1N1B4_4848
SM_b21106 ABC transporter for L-fucose, ATPase component Pf1N1B4_4847 Pf1N1B4_593

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory