Alignments for a candidate for HSERO_RS05250 in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS05250 in Pseudomonas fluorescens FW300-N1B4

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate Pf1N1B4_1243 ABC transporter ATP-binding protein

Query= uniprot:D8J111
         (520 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_1243
          Length = 521

 Score =  263 bits (673), Expect = 9e-75
 Identities = 167/493 (33%), Positives = 266/493 (53%), Gaps = 18/493 (3%)

Query: 20  VPVIALRNVCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDI 79
           +P + LR + KR+PG LA D     +A GE+HAL+GENGAGKSTLMKI+ GV   DSG++
Sbjct: 11  IPRLQLRRISKRYPGCLANDAIDLSIAPGEIHALLGENGAGKSTLMKIIYGVTHADSGEM 70

Query: 80  LLDGKPVEITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFI-DEDELN 138
           +  G+ V +  P QA+ LGIG++ Q  +L   LS AQNI +      AMG       +L 
Sbjct: 71  IWQGQRVTMRNPAQARGLGIGMVFQHFSLFETLSVAQNIAL------AMGAAAGTPKQLE 124

Query: 139 RQAAAIFARMRLDMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAE 198
            +   +  R  + ++P   V  L++  +Q VEI + L  D R+LI+DEPT+ L   E  E
Sbjct: 125 PKIREVSQRYGMALEPERLVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQEADE 184

Query: 199 LFRIIRDLQAQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVG 258
           LF  +R L A+G  I++ISHK+ E+R +    +V+R G+        + S   +  +MVG
Sbjct: 185 LFVTLRRLAAEGCSILFISHKLGEVRALCHSATVLRGGRVAGHCVPAQCSDRQLAQLMVG 244

Query: 259 RALDGEQRIPPDTSRNDVVLEVRGLN------RGRAIRDVSFTLRKGEILGFAGLMGAGR 312
            A +      P     D  L V GL+       G +++++ F +R GEI+G AG+ G G+
Sbjct: 245 EAAELITDY-PKVMGADAFLSVTGLSWHNPDPFGCSLKNIDFDVRSGEIVGVAGVAGNGQ 303

Query: 313 TEVARAIFGAD--PLEAGEIIIHGGKAVIKSPADAVAH-GIGYLSEDRKHFGLAVGMDVQ 369
            E+   + G +  P +    I  GG+ V     DA    G+ ++  +R   G    + + 
Sbjct: 304 DELLALLSGEERLPRDDSATISFGGQPVAHLRPDARRKLGLAFVPAERLGHGAVPELSLA 363

Query: 370 ANIALSSMGR-FTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAK 428
            N  L++  +     G + +  ++  A+  +R+  +KTP     AR LSGGN QK ++ +
Sbjct: 364 DNALLTAFQQGLVSHGLVQRGKVQALAEDIIRRFGVKTPDSLAPARSLSGGNLQKFILGR 423

Query: 429 WLLRDCDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVM 488
            +L+   +L    PT G+DVGA + I++ L AL + G AI++IS +L E+ ++  R+  +
Sbjct: 424 EILQQPKLLVAAHPTWGVDVGAAATIHRALIALRDAGAAILVISEDLDELFQICDRLGAL 483

Query: 489 CEGRITGELARAD 501
           C G+++   A  D
Sbjct: 484 CGGQLSPLQATVD 496



 Score = 98.6 bits (244), Expect = 5e-25
 Identities = 67/219 (30%), Positives = 109/219 (49%), Gaps = 9/219 (4%)

Query: 292 VSFTLRKGEILGFAGLMGAGRTEVARAIFGADPLEAGEIIIHGGKAVIKSPADAVAHGIG 351
           +  ++  GEI    G  GAG++ + + I+G    ++GE+I  G +  +++PA A   GIG
Sbjct: 32  IDLSIAPGEIHALLGENGAGKSTLMKIIYGVTHADSGEMIWQGQRVTMRNPAQARGLGIG 91

Query: 352 YLSEDRKHFGLAVGMDVQANIALSSMGRFTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQ 411
            + +   HF L   + V  NIAL+ MG         +  IRE +Q Y   L       E+
Sbjct: 92  MVFQ---HFSLFETLSVAQNIALA-MGAAAGTPKQLEPKIREVSQRYGMAL-----EPER 142

Query: 412 QARLLSGGNQQKIVIAKWLLRDCDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMI 471
               LS G +Q++ I + L++D  +L  DEPT  +      E++  L  LA +G +I+ I
Sbjct: 143 LVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQEADELFVTLRRLAAEGCSILFI 202

Query: 472 SSELPEVLRMSHRVLVMCEGRITGELARADATQEKIMQL 510
           S +L EV  + H   V+  GR+ G    A  +  ++ QL
Sbjct: 203 SHKLGEVRALCHSATVLRGGRVAGHCVPAQCSDRQLAQL 241



 Score = 63.2 bits (152), Expect = 2e-14
 Identities = 56/232 (24%), Positives = 101/232 (43%), Gaps = 10/232 (4%)

Query: 37  ALDNCQFELAAGEVHALMGENGAGKSTLMKILSG---VYQRDSGDILLDGKPVEITEPRQ 93
           +L N  F++ +GE+  + G  G G+  L+ +LSG   + + DS  I   G+PV    P  
Sbjct: 279 SLKNIDFDVRSGEIVGVAGVAGNGQDELLALLSGEERLPRDDSATISFGGQPVAHLRPDA 338

Query: 94  AQALGIGIIHQEL---NLMNHLSAAQNIFIG--REPRKAMGLFIDEDELNRQAAAIFARM 148
            + LG+  +  E      +  LS A N  +   ++   + GL +   ++   A  I  R 
Sbjct: 339 RRKLGLAFVPAERLGHGAVPELSLADNALLTAFQQGLVSHGL-VQRGKVQALAEDIIRRF 397

Query: 149 RLDM-DPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRIIRDLQ 207
            +   D   P   L+    Q   + + +    ++L+   PT  ++    A + R +  L+
Sbjct: 398 GVKTPDSLAPARSLSGGNLQKFILGREILQQPKLLVAAHPTWGVDVGAAATIHRALIALR 457

Query: 208 AQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVGR 259
             G  I+ IS  +DEL QI DR+  +  G+        +T +  +   M G+
Sbjct: 458 DAGAAILVISEDLDELFQICDRLGALCGGQLSPLQATVDTHLSDVGGWMAGQ 509


Lambda     K      H
   0.320    0.135    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 611
Number of extensions: 48
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 520
Length of database: 521
Length adjustment: 35
Effective length of query: 485
Effective length of database: 486
Effective search space:   235710
Effective search space used:   235710
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources