GapMind for catabolism of small carbon sources

 

Aligments for a candidate for tpi in Pseudomonas fluorescens FW300-N1B4

Align triose-phosphate isomerase (EC 5.3.1.1) (characterized)
to candidate Pf1N1B4_2399 Phosphoglycerate kinase (EC 2.7.2.3)

Query= BRENDA::P36204
         (654 letters)



>lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2399 Phosphoglycerate
           kinase (EC 2.7.2.3)
          Length = 387

 Score =  327 bits (837), Expect = 8e-94
 Identities = 184/401 (45%), Positives = 256/401 (63%), Gaps = 17/401 (4%)

Query: 1   MEKMTIRDVDLKGKRVIMRVDFNVPVKDGVVQDDTRIRAALPTIKYALEQGAKVILLSHL 60
           M  + + D+DL+GKRV++R D NVPVKDGVV  D RI A+LPTIK ALE+GA V++ SHL
Sbjct: 1   MTVLKMTDLDLQGKRVLIREDLNVPVKDGVVTSDARILASLPTIKLALEKGAAVMICSHL 60

Query: 61  GRP-KGEPSPEFSLAPVAKRLSELLGKEVKFVPAVVGDEVKKAVEELKEGEVLLLENTRF 119
           GRP +GE S E SL PVA  LS+ LG+EV  V   +G        ++K G+++L EN RF
Sbjct: 61  GRPTEGEFSAENSLKPVADYLSKALGREVPLVSDYLGG------VDVKAGDIVLFENVRF 114

Query: 120 HPGETKNDPELAKFWASLADIHVNDAFGTAHRAHASNVGIAQFIP-SVAGFLMEKEIKFL 178
           + GE KN  ELAK +A+L D+ V DAFGTAHRA  S  G+A+F   + AG L+  E+  L
Sbjct: 115 NKGEKKNADELAKQYAALCDVFVMDAFGTAHRAEGSTHGVAKFAKVAAAGPLLAAELDAL 174

Query: 179 SKVTYNPEKPYVVVLGGAKVSDKIGVITNLMEKADRILIGGAMMFTFLKALGKEVGSSRV 238
            K    P +P   ++ G+KVS K+ V+ +L +  +++++GG +  TFL A G  VG S  
Sbjct: 175 GKALGAPAQPMAAIVAGSKVSTKLDVLNSLSQICNQLIVGGGIANTFLAAAGHPVGKSLY 234

Query: 239 EEDKIDLAKELLEKAKEKGVEIVLPVDAVIAQKIEPGVEKKVVRIDDGIPEGWMGLDIGP 298
           E D +D A+E+  K     V + LPVD V+A++        V  I D +    M LDIGP
Sbjct: 235 EPDLLDTAREIAAK-----VSVPLPVDVVVAKEFAESATATVKLIAD-VAADDMILDIGP 288

Query: 299 ETIELFKQKLSDAKTVVWNGPMGVFEIDDFAEGTKQVALAIAALTEKGAITVVGGGDSAA 358
           +T   F + L  +KT++WNGP+GVFE D F  GTK +A AIA   E  A ++ GGGD+ A
Sbjct: 289 QTAANFAELLKSSKTILWNGPVGVFEFDQFGNGTKVLAQAIA---ESSAFSIAGGGDTLA 345

Query: 359 AVNKFGLEDKFSHVSTGGGASLEFLEGKELPGIASIADKKK 399
           A++K+G+ ++ S++STGGGA LEF+EGK LP +  +  + K
Sbjct: 346 AIDKYGVAEQISYISTGGGAFLEFVEGKVLPAVEVLESRAK 386


Lambda     K      H
   0.317    0.137    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 568
Number of extensions: 33
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 654
Length of database: 387
Length adjustment: 34
Effective length of query: 620
Effective length of database: 353
Effective search space:   218860
Effective search space used:   218860
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory