GapMind for catabolism of small carbon sources

 

Alignments for a candidate for glucosaminate-lyase in Pseudomonas fluorescens FW300-N1B4

Align Glucosaminate ammonia-lyase; EC 4.3.1.9; D-glucosaminate dehydratase alpha-subunit; GlcNA-DH alpha subunit; GlcNADH-alpha (uncharacterized)
to candidate Pf1N1B4_1046 Thioredoxin reductase (EC 1.8.1.9)

Query= curated2:Q93HX6
         (320 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_1046
          Length = 318

 Score =  612 bits (1578), Expect = e-180
 Identities = 307/318 (96%), Positives = 312/318 (98%)

Query: 1   MVEVRHSRVIILGSGPAGYSAAVYAARANLKPLLITGMQAGGQLTTTTEVDNWPGDVHGL 60
           M EVRHSRVIILGSGPAGYSAAVYAARANLKPLLITGMQAGGQLTTTTEVDNWPGDVHGL
Sbjct: 1   MSEVRHSRVIILGSGPAGYSAAVYAARANLKPLLITGMQAGGQLTTTTEVDNWPGDVHGL 60

Query: 61  TGPALMERMREHAERFETEIVFDHINAVDFAAKPYTLTGDSATYTCDALIIATGASARYL 120
           TGPALMERM+EHAERFETEIVFDHINAVDFAAKPYTL GDSATYTCDALIIATGASARYL
Sbjct: 61  TGPALMERMKEHAERFETEIVFDHINAVDFAAKPYTLIGDSATYTCDALIIATGASARYL 120

Query: 121 GLPSEEAFMGKGVSACATCDGFFYRNKPVAVVGGGNTAVEEALYLANIASTVTLIHRRET 180
           GLPSEE FMGKGVSACATCDGFFYRNKPVAVVGGGNTAVEEALYLANIASTVTLIHRRET
Sbjct: 121 GLPSEETFMGKGVSACATCDGFFYRNKPVAVVGGGNTAVEEALYLANIASTVTLIHRRET 180

Query: 181 FRAEKILIDKLNARVAEGKIILKLNANLDEVLGDNMGVTGARLKNNDGSFDELKVDGVFI 240
           FRAEKILIDKLNARVAEGKI+LKLN+ LDEVLGDNMGVTGARLKNNDGSFDELKVDGVFI
Sbjct: 181 FRAEKILIDKLNARVAEGKIVLKLNSTLDEVLGDNMGVTGARLKNNDGSFDELKVDGVFI 240

Query: 241 AIGHTPNTSLFEGQLTLKDGYLVVQGGRDGNATATSVEGIFAAGDVADHVYRQAITSAGA 300
           AIGHTPNTSLFEGQLTLKDGYLVVQGGRDGNATAT++EGIFAAGDVADHVYRQAITSAGA
Sbjct: 241 AIGHTPNTSLFEGQLTLKDGYLVVQGGRDGNATATNLEGIFAAGDVADHVYRQAITSAGA 300

Query: 301 GCMAALDTERYLDGLQNA 318
           GCMAALD ERYLD LQNA
Sbjct: 301 GCMAALDAERYLDDLQNA 318


Lambda     K      H
   0.318    0.135    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 495
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 320
Length of database: 318
Length adjustment: 28
Effective length of query: 292
Effective length of database: 290
Effective search space:    84680
Effective search space used:    84680
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory