GapMind for catabolism of small carbon sources

 

Alignments for a candidate for BPHYT_RS24010 in Pseudomonas fluorescens FW300-N1B4

Align Polar amino acid ABC transporter, inner membrane subunit (characterized, see rationale)
to candidate Pf1N1B4_1643 Amino acid ABC transporter, permease protein

Query= uniprot:B2TBJ8
         (250 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_1643
          Length = 229

 Score =  177 bits (450), Expect = 1e-49
 Identities = 91/217 (41%), Positives = 136/217 (62%)

Query: 4   DFDFLFDTIKQLLAAVPTTLGLFFCSLILGGLLSLVIVTMRVSPHWLPNRFARAYILVFR 63
           +++ +   + +L      TL L   ++I G LL++ +   R S  W    F   YI  FR
Sbjct: 2   NWEVIIKWLPKLAQGATLTLELVAIAVIAGLLLAIPLGIARSSRLWYVRAFPYGYIFFFR 61

Query: 64  GSPLLIQMFLVYYGMGQFGVIRESFLWPVLREPYMCAVLSLALCTAGYTAEIIRGGLMAV 123
           G+PLL+Q+FLVYYG+ QF  +R S +WP LR P+ CA  ++ L TA Y AEI+RG + A+
Sbjct: 62  GTPLLVQLFLVYYGLAQFDAVRNSSMWPYLRSPFWCATATMTLHTAAYIAEILRGAIQAI 121

Query: 124 PVGQIEAGYSIGLSGFALLRRVIGPIALRQCLPAYSTEAVLLVKSTALASLVTVWEVTGV 183
           P G+IEA  ++G+S    L  ++ P A R  LPAYS E +L++K++ALAS VT+ E+TG+
Sbjct: 122 PPGEIEAARALGMSRTKALFYIMLPRAARIGLPAYSNEVILMLKASALASTVTLLELTGM 181

Query: 184 AQQIIQQTYRTTEVFICAALIYLFLNFVIVRLLGMLE 220
           A+ II +TY   E+F  A + YL + +V+VR   +LE
Sbjct: 182 ARTIIARTYLPVEIFFAAGMFYLLMAYVLVRGFKLLE 218


Lambda     K      H
   0.331    0.143    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 147
Number of extensions: 3
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 250
Length of database: 229
Length adjustment: 23
Effective length of query: 227
Effective length of database: 206
Effective search space:    46762
Effective search space used:    46762
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory