Align ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, permease component 2 (characterized)
to candidate Pf1N1B4_915 Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4)
Query= reanno::Smeli:SMc02120 (384 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_915 Length = 365 Score = 413 bits (1061), Expect = e-120 Identities = 206/367 (56%), Positives = 257/367 (70%), Gaps = 13/367 (3%) Query: 18 PAPSLESGAVSWLRKNLFATPKDTALTIISLLILAWLVPPAIQWLFIDAAWSGGGRGVCA 77 P PS G V+W+R ++F++ +T LT+ + ++ +VPP + W +DA W G R C Sbjct: 11 PPPSSSIGVVAWMRAHMFSSWINTLLTLFAFYLIYLIVPPVVSWAILDANWVGTTRADCT 70 Query: 78 TLSQGGSQPEGWSGACWAFVNAKFAQFLFGRYPLDERWRPALVGILFVLLLVPMLIPRIP 137 GACW F+ +F QF++G YP D RWR L L V+ + P+ I R+P Sbjct: 71 K-----------EGACWVFIQQRFGQFMYGYYPTDLRWRVDLTVWLAVIGVAPLFIARVP 119 Query: 138 YKGLNALLLLVALPILSAILLPGGWFGLTYVETPLWGGLMVTLVLSFVGIAVSLPLGILL 197 +K + L LV PI+S +LL GGWFGLT V T WGGLM+TLV++ VGI +LPLGI+L Sbjct: 120 HKAIYGLSFLVLYPIISYVLLHGGWFGLTEVATSQWGGLMLTLVIATVGIVGALPLGIVL 179 Query: 198 ALGRRSNMPVIKMLCTVFIEVIRGVPLITVLFMASVMLPLFLPQGVTFDKFLRALIGVSL 257 ALGRRSNMP I+++C FIE RGVPLITVLFM+SVMLPLFLP+G+ FDK LRALIGV L Sbjct: 180 ALGRRSNMPAIRVVCVTFIEFWRGVPLITVLFMSSVMLPLFLPEGMNFDKLLRALIGVIL 239 Query: 258 FASAYMAEVVRGGLQAIPKGQYEGADSLGLSFWQKMGFIVLPQALKLVIPGIVNTFIGLF 317 F SAY+AEVVRGGLQAIPKGQYE A ++GL +W+ MG ++LPQALKLVIPGIVNTFI LF Sbjct: 240 FQSAYIAEVVRGGLQAIPKGQYEAAAAMGLGYWRSMGLVILPQALKLVIPGIVNTFIALF 299 Query: 318 KDTSLVSIIGMFDLLGIVRLNFSDTNWATAVTPLTGLIFAGFVFWLFCFGMSRYSGFMER 377 KDTSLV IIG+FDLL V+ +D W T G +FA VFW+FCFGMSRYS +ER Sbjct: 300 KDTSLVIIIGLFDLLNSVKQAAADPKWLGMAT--EGYVFAALVFWIFCFGMSRYSMHLER 357 Query: 378 LLDRSQR 384 LD + Sbjct: 358 KLDTGHK 364 Lambda K H 0.329 0.144 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 582 Number of extensions: 26 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 384 Length of database: 365 Length adjustment: 30 Effective length of query: 354 Effective length of database: 335 Effective search space: 118590 Effective search space used: 118590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory