Alignments for a candidate for brnQ in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for brnQ in Pseudomonas fluorescens FW300-N1B4

Align Branched chain amino acid transporter 2 of 439 aas and 12 TMSs, BrnQ (characterized)
to candidate Pf1N1B4_3686 Branched-chain amino acid transport system carrier protein

Query= TCDB::P0AD99
         (439 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3686
          Length = 437

 Score =  588 bits (1515), Expect = e-172
 Identities = 288/429 (67%), Positives = 351/429 (81%)

Query: 5   LRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTAAFGFLITAVGLPVLTVVALA 64
           L+ +DI+ALGFMTFALFVGAGNIIFPP+VGLQ+G +VW AA GFLITAVGLPV+TVVALA
Sbjct: 4   LKGQDILALGFMTFALFVGAGNIIFPPIVGLQSGPNVWMAALGFLITAVGLPVITVVALA 63

Query: 65  KVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDSALPLFI 124
           KVGG +D+LS+PIGKVAG LLA  CYLAVGPLFATPRTATVSFEVG+APLTG+S L LF+
Sbjct: 64  KVGGAMDALSSPIGKVAGGLLAAACYLAVGPLFATPRTATVSFEVGLAPLTGESPLALFL 123

Query: 125 YSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALVILSVAAIVWPAGSISTATEAYQNA 184
           YS VYF +V  +SLYPG+LLDTVG FLAPLKIIAL +L +AA   PAG I  AT  Y  A
Sbjct: 124 YSSVYFLVVFFISLYPGRLLDTVGRFLAPLKIIALAVLGIAAFALPAGDIGVATPEYVAA 183

Query: 185 AFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGVTEARLLTRYTVWAGLMAGVGLTLLYL 244
            FS GF+NGYLTMDTLGA+VFGIVIVNA RSRGV    L+TRY + AGL+AGVGL L+Y+
Sbjct: 184 PFSQGFINGYLTMDTLGALVFGIVIVNAIRSRGVESPALITRYAIIAGLIAGVGLALVYV 243

Query: 245 ALFRLGSDSASLVDQSANGAAILHAYVQHTFGGGGSFLLAALIFIACLVTAVGLTCACAE 304
           +LFRLGS S  +   +ANGAA+LHAYVQHTFG  GS  LA LI +ACLVTAVGLTCACAE
Sbjct: 244 SLFRLGSGSHEVAAGAANGAAVLHAYVQHTFGSLGSGFLAVLISLACLVTAVGLTCACAE 303

Query: 305 FFAQYVPLSYRTLVFILGGFSMVVSNLGLSQLIQISVPVLTAIYPPCIALVVLSFTRSWW 364
           +F++ +PLSY+TLV IL  FS++VSNLGL++LI  S+PVLTAIYPPCIALV LSF + +W
Sbjct: 304 YFSRVLPLSYKTLVIILAAFSLLVSNLGLTKLIAFSIPVLTAIYPPCIALVALSFCKDFW 363

Query: 365 HNSSRVIAPPMFISLLFGILDGIKASAFSDILPSWAQRLPLAEQGLAWLMPTVVMVVLAI 424
           H   R++ P M +S +FG++D +K +  +D +P+    LPL+EQGLAWL+P+V+ +V+A+
Sbjct: 364 HEQGRIVGPVMLVSFIFGLIDALKGAGLADWMPTQLAHLPLSEQGLAWLVPSVMTLVVAV 423

Query: 425 IWDRAAGRQ 433
           + DR  G++
Sbjct: 424 VCDRLLGKR 432


Lambda     K      H
   0.327    0.140    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 697
Number of extensions: 23
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 439
Length of database: 437
Length adjustment: 32
Effective length of query: 407
Effective length of database: 405
Effective search space:   164835
Effective search space used:   164835
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources