GapMind for catabolism of small carbon sources

 

D-mannitol catabolism in Pseudomonas fluorescens FW300-N1B4

Best path

mtlE, mtlF, mtlG, mtlK, mt2d, scrK

Also see fitness data for the top candidates

Rules

Overview: Mannitol degradation in GapMind is based on MetaCyc pathway mannitol degradation I via a phosphotransferase system (link), pathway II via mannitol 1-dehydrogenase (link), or another oxidative pathway with mannitol 2-dehydrogenase (PMID:8254318).

18 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
mtlE polyol ABC transporter, substrate-binding component MtlE/SmoE Pf1N1B4_4850 Pf1N1B4_5038
mtlF polyol ABC transporter, permease component 1 (MtlF/SmoF) Pf1N1B4_4849 Pf1N1B4_5113
mtlG polyol ABC transporter, permease component 2 (MtlG/SmoG) Pf1N1B4_4848 Pf1N1B4_5114
mtlK polyol ABC transporter, ATP component MtlK/SmoG Pf1N1B4_4847 Pf1N1B4_5115
mt2d mannitol 2-dehydrogenase Pf1N1B4_4846 Pf1N1B4_4527
scrK fructokinase Pf1N1B4_4844 Pf1N1B4_4765
Alternative steps:
cmtA mannitol phosphotransferase system, EII-CB component CmtA/MtlF
cmtB mannitol phosphotransferase system, EII-A component CmtB/MtlF Pf1N1B4_1146
gutA mannitol PTS system, EII-C2 component GutA
gutB mannitol PTS system, EII-A component GutB
gutE mannitol PTS system, EII-BC1 component GutE
mak mannose kinase
manA mannose-6-phosphate isomerase Pf1N1B4_993 Pf1N1B4_1964
mt1d mannitol 1-dehydrogenase Pf1N1B4_395
mtlA mannitol phosphotransferase system, EII-CBA components
mtlD mannitol-1-phosphate 5-dehydrogenase Pf1N1B4_4846
mtlP mannitol transporter, MFS type Pf1N1B4_397 Pf1N1B4_3380
PLT5 polyol transporter PLT5

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory