Alignments for a candidate for mt2d in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for mt2d in Pseudomonas fluorescens FW300-N1B4

Align mannitol 2-dehydrogenase (EC 1.1.1.67) (characterized)
to candidate Pf1N1B4_4846 Multiple polyol-specific dehydrogenase (EC 1.1.1.-)

Query= BRENDA::O08355
         (493 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4846
          Length = 491

 Score =  871 bits (2250), Expect = 0.0
 Identities = 425/489 (86%), Positives = 452/489 (92%)

Query: 1   MKLNKQNLTQLAPEVKLPAYTLADTRQGIAHIGVGGFHRAHQAYYTDALMNTGEGLDWSI 60
           MKLNKQNL +LAPEV LPAYTL+DTRQGIAHIGVGGFHRAHQAYYTDALMNTGE LDW+I
Sbjct: 1   MKLNKQNLNRLAPEVALPAYTLSDTRQGIAHIGVGGFHRAHQAYYTDALMNTGEALDWAI 60

Query: 61  CGVGLRSEDRKARDDLAGQDYLFTLYELGDTDDTEVRVIGSISDMLLAEDSAQALIDKLA 120
           CGVGLR+EDR+ARDDL  QDYLFTL+ELGD DD EVRVIG+I DMLLAEDS + LIDKLA
Sbjct: 61  CGVGLRAEDRRARDDLKEQDYLFTLFELGDADDAEVRVIGAIRDMLLAEDSPEVLIDKLA 120

Query: 121 SPEIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLAHPSSPKTVFGFICAALTQRRAAG 180
            P+IRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLAHP++PKTVFGF+CAAL +RRAAG
Sbjct: 121 DPDIRIVSLTITEGGYCIDDSNGEFMAHLPQIQHDLAHPNAPKTVFGFLCAALAKRRAAG 180

Query: 181 IPAFTVMSCDNLPHNGAVTRKALLAFAALHNAELHDWIKAHVSFPNAMVDRITPMTSTAH 240
            PAFT+MSCDNLPHNGAVTRKALLAFAAL +A+L DWI+ HVSFPNAMVDRITPMTST H
Sbjct: 181 TPAFTLMSCDNLPHNGAVTRKALLAFAALRDADLRDWIERHVSFPNAMVDRITPMTSTEH 240

Query: 241 RLQLHDEHGIDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVTPYEEMKIGLLN 300
           RLQLHD+H IDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVTPYEEMKI LLN
Sbjct: 241 RLQLHDKHSIDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVTPYEEMKIKLLN 300

Query: 301 GSHLALTYLGFLKGYRFVHETMNDPLFVAYMRAYMDLDVTPNLAPVPGIDLTDYKQTLVD 360
           GSHLALTYLGFLKGYRFVHETMNDPLFV YMRAYMDLDVTP LAPVPGIDLT+YK TLV 
Sbjct: 301 GSHLALTYLGFLKGYRFVHETMNDPLFVRYMRAYMDLDVTPQLAPVPGIDLTEYKNTLVA 360

Query: 361 RFSNQAIADQLERVCSDGSSKFPKFTVPTINRLIADGRETERAALVVAAWALYLKGVDEN 420
           RFSNQAIADQLERVCSDGSSKFPKFTVPTIN LIADG+ET+RAALVVAAWALYLKGVDEN
Sbjct: 361 RFSNQAIADQLERVCSDGSSKFPKFTVPTINCLIADGQETKRAALVVAAWALYLKGVDEN 420

Query: 421 GVSYTIPDPRAEFCQGLVSDDALISQRLLAVEEIFGTAIPNSPEFVAAFERCYGSLRDNG 480
           G +Y IPDPRA FCQ LV+DD LI+QRLL VEEIFGTAIP SPEFVAAFE C  SLR+ G
Sbjct: 421 GQTYAIPDPRAAFCQALVADDVLITQRLLEVEEIFGTAIPRSPEFVAAFEWCCNSLREVG 480

Query: 481 VTTTLKHLL 489
           VT TL+ +L
Sbjct: 481 VTRTLERVL 489


Lambda     K      H
   0.321    0.137    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 957
Number of extensions: 29
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 493
Length of database: 491
Length adjustment: 34
Effective length of query: 459
Effective length of database: 457
Effective search space:   209763
Effective search space used:   209763
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources