Align ABC transporter for D-mannitol and D-mannose, permease component 2 (characterized)
to candidate Pf1N1B4_4848 Various polyols ABC transporter, permease component 2
Query= reanno::pseudo3_N2E3:AO353_25890 (276 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4848 Length = 276 Score = 539 bits (1389), Expect = e-158 Identities = 271/276 (98%), Positives = 275/276 (99%) Query: 1 MTLQQTRRLQSLLLGTLAWAIAILIFFPIFWMVLTSFKTEIDAFATPPQFIFTPTLENYL 60 MTLQQ+RRLQSLLLGTLAWAIAILIFFPIFWMVLTSFKTEIDAFATPPQFIFTPTLENYL Sbjct: 1 MTLQQSRRLQSLLLGTLAWAIAILIFFPIFWMVLTSFKTEIDAFATPPQFIFTPTLENYL 60 Query: 61 HVNERSGYFSFAWNSVVISFSATALCLLIAVPAAYSMAFYETQRTKGTLLWMLSTKMLPP 120 H+NERS YFSFAWNSVVISFSATALCLLIAVPAAYSMAFYETQRTKGTLLWMLSTKMLPP Sbjct: 61 HINERSDYFSFAWNSVVISFSATALCLLIAVPAAYSMAFYETQRTKGTLLWMLSTKMLPP 120 Query: 121 VGVLMPIYLLAKSFGLLDTRIALIIIYTLINLPIVVWMIYTYFKDIPKDILEAARLDGAT 180 VGVLMPIYLLAKSFGLLDTRIALI+IYTLINLPIVVWMIYTYFKDIPKDILEAARLDGAT Sbjct: 121 VGVLMPIYLLAKSFGLLDTRIALIVIYTLINLPIVVWMIYTYFKDIPKDILEAARLDGAT 180 Query: 181 LWQEMVRVLLPIAKGGLASTVLLSLILCWNEAFWSLNLTSSKAAPLTALIASYSSPEGLF 240 LWQEMVRVLLPIAKGGLASTVLLSLILCWNEAFWSLNLTSS+AAPLTALIASYSSPEGLF Sbjct: 181 LWQEMVRVLLPIAKGGLASTVLLSLILCWNEAFWSLNLTSSQAAPLTALIASYSSPEGLF 240 Query: 241 WAKLSAVSTLACAPILIFGWISQKQLVRGLSFGAVK 276 WAKLSAVSTLACAPILIFGWISQKQLVRGLSFGAVK Sbjct: 241 WAKLSAVSTLACAPILIFGWISQKQLVRGLSFGAVK 276 Lambda K H 0.327 0.138 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 475 Number of extensions: 10 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 276 Length of database: 276 Length adjustment: 25 Effective length of query: 251 Effective length of database: 251 Effective search space: 63001 Effective search space used: 63001 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory