GapMind for catabolism of small carbon sources

 

Aligments for a candidate for paaG in Pseudomonas fluorescens FW300-N1B4

Align 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized)
to candidate Pf1N1B4_679 Enoyl-CoA hydratase (EC 4.2.1.17)

Query= BRENDA::Q5SLK3
         (254 letters)



>lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_679 Enoyl-CoA hydratase
           (EC 4.2.1.17)
          Length = 249

 Score =  121 bits (304), Expect = 1e-32
 Identities = 80/240 (33%), Positives = 123/240 (51%), Gaps = 3/240 (1%)

Query: 5   ERQDGVLVLTLNRPEKLNAITGELLDALYAALKEGEEDREVRALLLTGAGRAFSAGQDLT 64
           ER+ G+L L +NRPEK NA+T  +   L   L+  + D E+ A+L+TG+   F+AG D+ 
Sbjct: 8   ERERGLLTLRINRPEKKNALTRAMYSQLAEGLRLADSDPEINAVLITGSAECFTAGNDIF 67

Query: 65  EFGDRKPDYEAHLRRYNRVVEALSGLEKPLVVAVNGVAAGAGMSLALWGDLRLAAVGASF 124
           +F  + P        Y+ ++  L    KP++ AV G A G G +L L  DL   +  A  
Sbjct: 68  DFLQQPPSI-LDSPVYHFMLNLLE-CRKPVIAAVAGAAVGIGTTLLLHCDLVYVSTDARL 125

Query: 125 TTAFVRIGLVPDSGLSFLLPRLVGLAKAQELLLLSPRLSAEEALALGLVHRVV-PAEKLM 183
              FV +GL P+ G S +LPR++G AKA ELLLL    S E+A A G+    +   E  +
Sbjct: 126 RMPFVNLGLCPEFGSSLILPRMLGHAKAAELLLLGEGFSGEQAAAWGIATEALGSGEAAL 185

Query: 184 EEALSLAKELAQGPTRAYALTKKLLLETYRLSLTEALALEAVLQGQAGQTQDHEEGVRAF 243
            +A  +A      P  A  ++K+L+    R  L + +  E  L  Q  ++ +    + AF
Sbjct: 186 AKAREMALRFEALPPEAVRISKQLMKAPGREQLRKVIEEEGALFTQRLRSPEAMAALSAF 245


Lambda     K      H
   0.318    0.135    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 178
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 254
Length of database: 249
Length adjustment: 24
Effective length of query: 230
Effective length of database: 225
Effective search space:    51750
Effective search space used:    51750
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory