Align aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)
to candidate Pf1N1B4_2132 Aldehyde dehydrogenase (EC 1.2.1.3)
Query= BRENDA::P05091 (517 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2132 Length = 497 Score = 403 bits (1036), Expect = e-117 Identities = 210/476 (44%), Positives = 298/476 (62%), Gaps = 4/476 (0%) Query: 40 FINNEWHDAVSRKTFPTVNPSTGEVICQVAEGDKEDVDKAVKAARAAFQLGSPWRRMDAS 99 +IN E+ DAVS +TF ++P G ++ ++A D D +AV ARA F G+ W R+ + Sbjct: 23 YINGEYTDAVSGETFECISPVDGRLLGKIASCDAADAQRAVDNARATFNSGA-WSRLAPT 81 Query: 100 HRGRLLNRLADLIERDRTYLAALETLDNGKPYVISYLVDLDMVLKCLRYYAGWADKYHGK 159 R + R A L+++ LA LETLD GKP S +D+ + L + DK + + Sbjct: 82 KRKATMIRFAGLLKQHAEELALLETLDMGKPISDSLYIDVPGAAQALSWSGEAIDKIYDE 141 Query: 160 TIPIDGDFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTAL 219 D EPVGV G I+PWNFPL+M WKLGPAL+TGN V++K +E++PLTA+ Sbjct: 142 VAATPHDQLGLVTREPVGVVGAIVPWNFPLMMACWKLGPALSTGNSVILKPSEKSPLTAI 201 Query: 220 YVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNL 279 +A L EAG P GV+N++PG+G T G A+A H DVD + FTGST+I + + + +G SN+ Sbjct: 202 RIAALAVEAGIPKGVLNVLPGYGHTVGKALALHNDVDTLVFTGSTKIAKQLLIYSGESNM 261 Query: 280 KRVTLELGGKSPNIIMSDA-DMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVER 338 KRV LE GGKSPNI+ +DA D+ A E A A+ FNQG+ C AGSR V+ I D+F+ Sbjct: 262 KRVWLEAGGKSPNIVFADAPDLQAAAEAAAGAIAFNQGEVCTAGSRLLVERSIKDKFLPL 321 Query: 339 SVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGG--IAADRGY 396 + K+ GNP D T G VD Q +L YI +G +GAKL+ GG + G Sbjct: 322 VIEALKTWKPGNPLDPATNVGALVDTQQMNTVLSYIESGHTDGAKLVAGGKRILQETGGT 381 Query: 397 FIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKA 456 +++PT+F V + M IA+EEIFGPV+ ++ F + EE + AN++ YGLAAAV+T D+ KA Sbjct: 382 YVEPTIFDGVSNAMKIAQEEIFGPVLSVITFDSAEEAIQIANDTPYGLAAAVWTADISKA 441 Query: 457 NYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKV 512 + ++AL+AG+VWVN YD +PFGG+K SG+GR+ + YTE+K +K+ Sbjct: 442 HLTAKALRAGSVWVNQYDGGDMTAPFGGFKQSGNGRDKSLHAFDKYTELKATWIKL 497 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 627 Number of extensions: 21 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 517 Length of database: 497 Length adjustment: 34 Effective length of query: 483 Effective length of database: 463 Effective search space: 223629 Effective search space used: 223629 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory