Alignments for a candidate for putP in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for putP in Pseudomonas fluorescens FW300-N1B4

Align L-proline uptake porter, PutP (characterized)
to candidate Pf1N1B4_1487 Proline/sodium symporter PutP (TC 2.A.21.2.1) @ Propionate/sodium symporter

Query= TCDB::Q9I5F5
         (506 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_1487
          Length = 494

 Score =  805 bits (2078), Expect = 0.0
 Identities = 409/493 (82%), Positives = 448/493 (90%), Gaps = 2/493 (0%)

Query: 1   MSVNTPTLITFVIYIAAMVLIGLAAYRSTNNFSDYILGGRSLGSFVTALSAGASDMSGWL 60
           MSV+ PTLITFVIYIAAMVLIG  AYRSTNN SDYILGGRSLGS VTALSAGASDMSGWL
Sbjct: 1   MSVSNPTLITFVIYIAAMVLIGFMAYRSTNNLSDYILGGRSLGSVVTALSAGASDMSGWL 60

Query: 61  LMGLPGAVYLSGLSESWIAIGLIVGAYLNWLFVAGRLRVQTEHNGNALTLPDYFTNRFED 120
           LMGLPGA+Y+SGLSESWIAIGLIVGAYLNWLFVAGRLRVQTEHNG+ALTLPDYF++RFED
Sbjct: 61  LMGLPGAIYMSGLSESWIAIGLIVGAYLNWLFVAGRLRVQTEHNGDALTLPDYFSSRFED 120

Query: 121 NSRLLRIFSALVILVFFTIYCASGIVAGARLFESTFGLSYETALWAGAAATIAYTFIGGF 180
            S LLRI SA+VILVFFTIYCASGIVAGARLFESTFG+SYETALWAGAAATIAYTF+GGF
Sbjct: 121 KSGLLRIISAVVILVFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATIAYTFVGGF 180

Query: 181 LAVSWTDTVQASLMIFALILTPVIVMLATGGVEPTFTAIELKDATSFDMLKGASFIGVIS 240
           LAVSWTDTVQA+LMIFAL+LTP+IV+LATGG++ TF AIE +D ++FDMLK  +FIG+IS
Sbjct: 181 LAVSWTDTVQATLMIFALLLTPIIVLLATGGIDTTFLAIEAQDPSNFDMLKNTTFIGIIS 240

Query: 241 LMAWGLGYFGQPHILARFMAADSVKSIPAARRISMTWMILCLGGAVAVGFFGIAYFQAHP 300
           LM WGLGYFGQPHILARFMAADSVKSI  ARRISMTWMILCLGG VAVGFFGIAYF AHP
Sbjct: 241 LMGWGLGYFGQPHILARFMAADSVKSIANARRISMTWMILCLGGTVAVGFFGIAYFSAHP 300

Query: 301 EQAGAVSENPERVFIELAKILFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKA 360
             A  VSEN ERVFIELAKILFNPW+AGVLLSAILAAVMSTLSCQLLVCSSALTEDFYK 
Sbjct: 301 AVAMPVSENHERVFIELAKILFNPWVAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKT 360

Query: 361 FLRKGASQLELVWVGRAMVLLVAVIAIWLASNPENRVLGLVSYAWAGFGAAFGPLVLFSL 420
           FLRK ASQ+ELVWVGRAMVLLVA+IAI +A+NPENRVLGLVSYAWAGFGAAFGP+VL S+
Sbjct: 361 FLRKSASQVELVWVGRAMVLLVALIAIAMAANPENRVLGLVSYAWAGFGAAFGPVVLISV 420

Query: 421 LWKRMTRNGALAGMIVGAATVILWKNLLGWTGLYEIIPGFLFASVAIVVFSLLGKAPSTS 480
           +WK MTRNGALAG++VGA TVI+WK+     GLYEIIPGF+FAS+AI + S LG  P+  
Sbjct: 421 IWKDMTRNGALAGILVGAITVIVWKH-FELLGLYEIIPGFIFASLAIYIVSKLG-TPTHG 478

Query: 481 MLKRFDDAEQEYR 493
           ML+RF  AE ++R
Sbjct: 479 MLQRFAAAEADFR 491


Lambda     K      H
   0.326    0.138    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 957
Number of extensions: 30
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 506
Length of database: 494
Length adjustment: 34
Effective length of query: 472
Effective length of database: 460
Effective search space:   217120
Effective search space used:   217120
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources