GapMind for catabolism of small carbon sources

 

Finding step potA for putrescine catabolism in Pseudomonas fluorescens FW300-N1B4

5 candidates for potA: putrescine ABC transporter, ATPase component (PotA/PotG)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi Pf1N1B4_4352 Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2) PotG aka B0855, component of Putrescine porter (characterized) 61% 96% 424.9 MalK aka PF1933, component of Maltooligosaccharide porter (Maltose is not a substrate, but maltotriose is.) 42% 255.0
hi Pf1N1B4_2261 Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2) PotG aka B0855, component of Putrescine porter (characterized) 62% 96% 421.4 MalK aka PF1933, component of Maltooligosaccharide porter (Maltose is not a substrate, but maltotriose is.) 40% 248.4
med Pf1N1B4_2538 Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) PotG aka B0855, component of Putrescine porter (characterized) 45% 93% 269.6 Putative ABC transporter component, component of The γ-aminobutyrate (GABA) uptake system, GtsABCD 47% 313.5
med Pf1N1B4_3027 Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) 42% 96% 267.3 Uncharacterized ABC transporter ATP-binding protein YdcT 67% 438.7
med Pf1N1B4_3744 ABC transporter, ATP-binding protein spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) 46% 78% 266.9 CP4-6 prophage; ABC transporter ATP-binding protein AfuC 42% 256.1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step potA

Or cluster all characterized potA proteins

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory