GapMind for catabolism of small carbon sources


L-serine catabolism in Pseudomonas fluorescens FW300-N1B4

Best path

braC, braD, braE, braF, braG, sdaB

Also see fitness data for the top candidates


Overview: L-serine degradation in GapMind is based on the MetaCyc pathway (link)

19 steps (16 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
braC L-alanine/L-serine/L-threonine ABC transporter, substrate binding protein (BraC/NatB) Pf1N1B4_3218 Pf1N1B4_1382
braD L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) Pf1N1B4_3217 Pf1N1B4_1381
braE L-alanine/L-serine/L-threonine ABC transporter, permease component 2 (BraE/NatC) Pf1N1B4_3216 Pf1N1B4_1380
braF L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 1 (BraF/NatA) Pf1N1B4_3215 Pf1N1B4_1346
braG L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 2 (BraG/NatE) Pf1N1B4_3214 Pf1N1B4_1378
sdaB L-serine ammonia-lyase Pf1N1B4_1013 Pf1N1B4_622
Alternative steps:
AAP1 L-serine transporter AAP1
Ac3H11_1692 L-tyrosine ABC transporter, ATPase component 2 Pf1N1B4_3214 Pf1N1B4_1378
Ac3H11_1693 L-tyrosine ABC transporter, ATPase component 1 Pf1N1B4_3215 Pf1N1B4_1379
Ac3H11_1694 L-tyrosine ABC transporter, permease component 2 Pf1N1B4_3216 Pf1N1B4_1380
Ac3H11_1695 L-tyrosine ABC transporter, permease component 1 Pf1N1B4_1381 Pf1N1B4_3217
Ac3H11_2396 L-tyrosine ABC transporter, substrate-binding component component Pf1N1B4_3218 Pf1N1B4_1382
dlsT L-serine transporter DlsT
sdaC L-serine transporter:H+ symporter sdaC Pf1N1B4_1014 Pf1N1B4_4562
sdhA FeS-containing L-serine dehydratase, alpha subunit Pf1N1B4_1013 Pf1N1B4_2423
sdhB FeS-containing L-serine dehydratase, beta subunit
serP L-serine permease SerP Pf1N1B4_4976 Pf1N1B4_1580
snatA L-serine transporter Pf1N1B4_1320
sstT L-serine:Na+ symporter SstT Pf1N1B4_3934

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.



Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory