Align Phosphogluconate dehydratase; EC 4.2.1.12; 6-phosphogluconate dehydratase (uncharacterized)
to candidate Pf1N1B4_2303 Dihydroxy-acid dehydratase (EC 4.2.1.9)
Query= curated2:P31961 (608 letters) >FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2303 Length = 613 Score = 222 bits (566), Expect = 3e-62 Identities = 170/543 (31%), Positives = 260/543 (47%), Gaps = 52/543 (9%) Query: 68 VAIVSAYNDMLSAHQPFERFPGLIKQALHEIGSVGQFAGGVPAMCDGVTQGEPGMELSLA 127 +AI +++ + H + L+ + + G V + + A+ DG+ G GM SL Sbjct: 37 IAIANSFTQFVPGHVHLKDLGQLVAREIERAGGVAKEFNTI-AVDDGIAMGHDGMLYSLP 95 Query: 128 SRDVIAMSTAIALSHNMFDAALCLGVCDKIVPGLLIGSLRFGHLPTVFVPAGPMPTGISN 187 SR++IA S ++ + DA +C+ CDKI PG+L+ SLR ++P +FV GPM G Sbjct: 96 SREIIADSVEYMVNAHCADAIVCISNCDKITPGMLMASLRL-NIPVIFVSGGPMEAG--- 151 Query: 188 KEKAAVRQL--------FAEGKATREELLASEMASYHAPGTCTFYGTANTNQLLVEVMGL 239 K K A L A+ A+ E++ E ++ G+C+ TAN+ LVE +GL Sbjct: 152 KTKLASHGLDLVDAMVIAADSSASDEKVAEYERSACPTCGSCSGMFTANSMNCLVEALGL 211 Query: 240 HLPGASFVNPNTPLRDELTREAARQ----ASRLTPENGNYVPMAEIVDEKAIVNSVVALL 295 LPG R++L +A R R EN V I + KA N++ + Sbjct: 212 ALPGNGSTLATHSDREQLFLQAGRTIVELCKRYYGENDESVLPRNIANFKAFENAMTLDI 271 Query: 296 ATGGSTNHTLHLLAIAQAAGIQLTWQDMSELSHVVPTLARIYPNGQA-DINHFQAAGGMS 354 A GGSTN LHLLA AQ A I +D+ LS VP L ++ PN Q + AGG+ Sbjct: 272 AMGGSTNTILHLLAAAQEAEIDFDLRDIDRLSRHVPQLCKVAPNIQKYHMEDVHRAGGIF 331 Query: 355 FLIRQLLDGGLLHEDVQTV----AGPGLRRY----------------------TREPFLE 388 ++ L GGLLH D+ TV G+ ++ T+ F + Sbjct: 332 SILGSLARGGLLHTDLPTVHSKSMAEGIAKWDITQTTDEAVHHFFKAGPAGIPTQTAFSQ 391 Query: 389 DGRLVWREGPERSLDEAILRPLDKPFSAEGGLRLMEGNLGRG--VMKVSAVAPEHQVVEA 446 R W E + + +R ++ +S EGGL ++ GN+ V+K + V V E Sbjct: 392 STR--W-ETLDDDRENGCIRSVEHAYSKEGGLAVLYGNIALDGCVVKTAGVDESIHVFEG 448 Query: 447 PVRIFHDQASLAAAFKAGELERDLVAVVRFQGPRAN-GMPELHKLTPFLGVLQDRGFKVA 505 +IF Q S A E++ + ++R++GP+ GM E+ T +L + G A Sbjct: 449 NAKIFESQDSAVRGILADEVKAGDIVIIRYEGPKGGPGMQEMLYPTSYL-KSKGLGKACA 507 Query: 506 LVTDGRMSGASGKVPAAIHVSPEAIAGGPLARLRDGDRVRVDGVNGELRVLVDDAEWQAR 565 L+TDGR SG + + H SPEA AGG + ++DGD+V +D N + +L+ D E AR Sbjct: 508 LLTDGRFSGGTSGLSIG-HASPEAAAGGAIGLVQDGDKVLIDIPNRSINLLISDEEMAAR 566 Query: 566 SLE 568 E Sbjct: 567 RAE 569 Lambda K H 0.319 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 858 Number of extensions: 51 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 608 Length of database: 613 Length adjustment: 37 Effective length of query: 571 Effective length of database: 576 Effective search space: 328896 Effective search space used: 328896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory