Alignments for a candidate for edd in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for edd in Pseudomonas fluorescens FW300-N1B4

Align Phosphogluconate dehydratase; EC 4.2.1.12; 6-phosphogluconate dehydratase (uncharacterized)
to candidate Pf1N1B4_2303 Dihydroxy-acid dehydratase (EC 4.2.1.9)

Query= curated2:P31961
         (608 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2303
          Length = 613

 Score =  222 bits (566), Expect = 3e-62
 Identities = 170/543 (31%), Positives = 260/543 (47%), Gaps = 52/543 (9%)

Query: 68  VAIVSAYNDMLSAHQPFERFPGLIKQALHEIGSVGQFAGGVPAMCDGVTQGEPGMELSLA 127
           +AI +++   +  H   +    L+ + +   G V +    + A+ DG+  G  GM  SL 
Sbjct: 37  IAIANSFTQFVPGHVHLKDLGQLVAREIERAGGVAKEFNTI-AVDDGIAMGHDGMLYSLP 95

Query: 128 SRDVIAMSTAIALSHNMFDAALCLGVCDKIVPGLLIGSLRFGHLPTVFVPAGPMPTGISN 187
           SR++IA S    ++ +  DA +C+  CDKI PG+L+ SLR  ++P +FV  GPM  G   
Sbjct: 96  SREIIADSVEYMVNAHCADAIVCISNCDKITPGMLMASLRL-NIPVIFVSGGPMEAG--- 151

Query: 188 KEKAAVRQL--------FAEGKATREELLASEMASYHAPGTCTFYGTANTNQLLVEVMGL 239
           K K A   L         A+  A+ E++   E ++    G+C+   TAN+   LVE +GL
Sbjct: 152 KTKLASHGLDLVDAMVIAADSSASDEKVAEYERSACPTCGSCSGMFTANSMNCLVEALGL 211

Query: 240 HLPGASFVNPNTPLRDELTREAARQ----ASRLTPENGNYVPMAEIVDEKAIVNSVVALL 295
            LPG          R++L  +A R       R   EN   V    I + KA  N++   +
Sbjct: 212 ALPGNGSTLATHSDREQLFLQAGRTIVELCKRYYGENDESVLPRNIANFKAFENAMTLDI 271

Query: 296 ATGGSTNHTLHLLAIAQAAGIQLTWQDMSELSHVVPTLARIYPNGQA-DINHFQAAGGMS 354
           A GGSTN  LHLLA AQ A I    +D+  LS  VP L ++ PN Q   +     AGG+ 
Sbjct: 272 AMGGSTNTILHLLAAAQEAEIDFDLRDIDRLSRHVPQLCKVAPNIQKYHMEDVHRAGGIF 331

Query: 355 FLIRQLLDGGLLHEDVQTV----AGPGLRRY----------------------TREPFLE 388
            ++  L  GGLLH D+ TV       G+ ++                      T+  F +
Sbjct: 332 SILGSLARGGLLHTDLPTVHSKSMAEGIAKWDITQTTDEAVHHFFKAGPAGIPTQTAFSQ 391

Query: 389 DGRLVWREGPERSLDEAILRPLDKPFSAEGGLRLMEGNLGRG--VMKVSAVAPEHQVVEA 446
             R  W E  +   +   +R ++  +S EGGL ++ GN+     V+K + V     V E 
Sbjct: 392 STR--W-ETLDDDRENGCIRSVEHAYSKEGGLAVLYGNIALDGCVVKTAGVDESIHVFEG 448

Query: 447 PVRIFHDQASLAAAFKAGELERDLVAVVRFQGPRAN-GMPELHKLTPFLGVLQDRGFKVA 505
             +IF  Q S      A E++   + ++R++GP+   GM E+   T +L   +  G   A
Sbjct: 449 NAKIFESQDSAVRGILADEVKAGDIVIIRYEGPKGGPGMQEMLYPTSYL-KSKGLGKACA 507

Query: 506 LVTDGRMSGASGKVPAAIHVSPEAIAGGPLARLRDGDRVRVDGVNGELRVLVDDAEWQAR 565
           L+TDGR SG +  +    H SPEA AGG +  ++DGD+V +D  N  + +L+ D E  AR
Sbjct: 508 LLTDGRFSGGTSGLSIG-HASPEAAAGGAIGLVQDGDKVLIDIPNRSINLLISDEEMAAR 566

Query: 566 SLE 568
             E
Sbjct: 567 RAE 569


Lambda     K      H
   0.319    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 858
Number of extensions: 51
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 608
Length of database: 613
Length adjustment: 37
Effective length of query: 571
Effective length of database: 576
Effective search space:   328896
Effective search space used:   328896
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources