Alignments for a candidate for treC in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for treC in Pseudomonas fluorescens FW300-N1B4

Align Alpha,alpha-phosphotrehalase (EC 3.2.1.93) (characterized)
to candidate Pf1N1B4_835 Trehalose-6-phosphate hydrolase (EC 3.2.1.93)

Query= reanno::pseudo3_N2E3:AO353_15985
         (549 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835
          Length = 549

 Score = 1050 bits (2714), Expect = 0.0
 Identities = 491/549 (89%), Positives = 517/549 (94%)

Query: 1   MQDWQRSVIYQIYPKSFHSHAGNPTGDLLGVVAKLDYLQWLGVDCLWITPFLRSPQRDNG 60
           MQDWQRSVIYQIYPKSFHSHAGNPTGDLLGVVAKLDYL WLGVDCLWITPFLRSPQRDNG
Sbjct: 1   MQDWQRSVIYQIYPKSFHSHAGNPTGDLLGVVAKLDYLHWLGVDCLWITPFLRSPQRDNG 60

Query: 61  YDISDYYAIDPSYGTMADCELLIAEAGKRGIKLMLDIVVNHTSIEHSWFQQARSSLDNPY 120
           YDISDYYAIDPSYGTMADCELLIAEAGKRGIKLMLDIVVNHTSIEH+WFQQARSSLDNPY
Sbjct: 61  YDISDYYAIDPSYGTMADCELLIAEAGKRGIKLMLDIVVNHTSIEHTWFQQARSSLDNPY 120

Query: 121 RDFYIWRDQPNNWESKFGGSAWEYEAQTGQYFLHLFDHTQADLNWDNPQVRAEVFKLMRF 180
           RDFYIWRDQPNNWESKFGGSAWEYEAQTGQY+LHLFDHTQADLNWDNPQVRAEVFK+MRF
Sbjct: 121 RDFYIWRDQPNNWESKFGGSAWEYEAQTGQYYLHLFDHTQADLNWDNPQVRAEVFKMMRF 180

Query: 181 WRDKGVGGFRLDVINLISKPADFPEDHTDGRRFYTDGPNVHEYLQEMHREVFEGHDLINV 240
           WRDKGVGGFRLDVINLISKPADFPED++DGRRFYTDGPNVHEYLQ+MHREVFEGHDLINV
Sbjct: 181 WRDKGVGGFRLDVINLISKPADFPEDNSDGRRFYTDGPNVHEYLQQMHREVFEGHDLINV 240

Query: 241 GEMSSTSLEHCIRYSRPDSKELSMTFNFHHLKVDYPNMQKWVRADFDFLELKRILSDWQT 300
           GEMSSTSLEHCIRYSRP+SKELSMTFNFHHLKVDYPN+QKWVRADFDFL LKRILSDWQT
Sbjct: 241 GEMSSTSLEHCIRYSRPESKELSMTFNFHHLKVDYPNLQKWVRADFDFLALKRILSDWQT 300

Query: 301 GMQAGGGWNALFWCNHDQPRVVSRFGDDGEHRVVSAKMLGTALHFLQGTPFIYQGEELGM 360
           GMQAGGGWNALFWCNHDQPRVVSRFG DGEHRVVSAKMLGTALHFLQGTPF+YQGEELGM
Sbjct: 301 GMQAGGGWNALFWCNHDQPRVVSRFGHDGEHRVVSAKMLGTALHFLQGTPFVYQGEELGM 360

Query: 361 TNPGFERIEQYRDVETLNIYRLKREAGESEASSMAAIMQKSRDNSRTPMQWSALPNAGFS 420
           TNPGF+ I+QYRDVETLNI+RLKREAG S+  +MAAIMQKSRDN RTPM W+  PNAGFS
Sbjct: 361 TNPGFDHIDQYRDVETLNIFRLKREAGSSDVDNMAAIMQKSRDNGRTPMHWNTEPNAGFS 420

Query: 421 SSEPWIGVPANAMQINVENQLDDTTSVLHHYRQLIALRRSEPLIQDGVYRQLLPTHKQVW 480
           + EPWIGVPANA QINV +QLDD  SVLHHYRQLIALRRSE L+ DGVYRQLLP H Q+W
Sbjct: 421 AVEPWIGVPANAAQINVAHQLDDPDSVLHHYRQLIALRRSETLMSDGVYRQLLPEHTQIW 480

Query: 481 VYLREGEGERLLVVNNFYGTACEVELPERVITDCMLQRLVISNYPDSEVRKQQLFLRPYE 540
            Y+REG+GERLLV+NNFYGT CEVELP  VI + M+Q LVISNYPD   R +Q+FLRPYE
Sbjct: 481 AYVREGQGERLLVLNNFYGTPCEVELPPEVINESMVQSLVISNYPDCPPRNRQVFLRPYE 540

Query: 541 SFVLHLTDH 549
           SFVL LTDH
Sbjct: 541 SFVLRLTDH 549


Lambda     K      H
   0.321    0.137    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1402
Number of extensions: 54
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 549
Length adjustment: 36
Effective length of query: 513
Effective length of database: 513
Effective search space:   263169
Effective search space used:   263169
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)

Align candidate Pf1N1B4_835 (Trehalose-6-phosphate hydrolase (EC 3.2.1.93))
to HMM TIGR02403 (treC: alpha,alpha-phosphotrehalase (EC 3.2.1.93))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR02403.hmm
# target sequence database:        /tmp/gapView.13366.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02403  [M=544]
Accession:   TIGR02403
Description: trehalose_treC: alpha,alpha-phosphotrehalase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                     Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                     -----------
   1.3e-261  854.9   0.0   1.5e-261  854.7   0.0    1.0  1  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835  Trehalose-6-phosphate hydrolase 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835  Trehalose-6-phosphate hydrolase (EC 3.2.1.93)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  854.7   0.0  1.5e-261  1.5e-261       2     543 ..       4     545 ..       3     546 .. 0.99

  Alignments for each domain:
  == domain 1  score: 854.7 bits;  conditional E-value: 1.5e-261
                                     TIGR02403   2 lkkkviyqiyPksfkdskgkgvGdlkGiiekldylkkLgvdviWltPiyksPqkDnGyDvsdyla 66 
                                                   ++++viyqiyPksf+++ g+ +Gdl G++ kldyl++Lgvd++W+tP+++sPq+DnGyD+sdy+a
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835   4 WQRSVIYQIYPKSFHSHAGNPTGDLLGVVAKLDYLHWLGVDCLWITPFLRSPQRDNGYDISDYYA 68 
                                                   99*************************************************************** PP

                                     TIGR02403  67 idpefGtledfeeLvkeakkrnikimlDlvlnhtsteheWfkkalag.dekyrdfyilrdekgkl 130
                                                   idp++Gt++d+e L++ea kr+ik+mlD+v+nhts+eh Wf++a+++ d++yrdfyi+rd    +
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835  69 IDPSYGTMADCELLIAEAGKRGIKLMLDIVVNHTSIEHTWFQQARSSlDNPYRDFYIWRD----Q 129
                                                   ***********************************************************5....6 PP

                                     TIGR02403 131 PtnWeskfGGsaWeleeetekyyLhLfdktqaDLnWeneevreevadvvkfwldkGvkGfrlDvi 195
                                                   P+nWeskfGGsaWe+e++t++yyLhLfd+tqaDLnW+n++vr+ev+++++fw+dkGv GfrlDvi
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835 130 PNNWESKFGGSAWEYEAQTGQYYLHLFDHTQADLNWDNPQVRAEVFKMMRFWRDKGVGGFRLDVI 194
                                                   9**************************************************************** PP

                                     TIGR02403 196 nliskdelfeddeegdgrkfytDgpkvheylkelneevfekdkdlltvGelssttiekcikyskl 260
                                                   nlisk+++f++d+ +dgr+fytDgp+vheyl+++++evfe  +dl++vGe+sst++e+ci+ys++
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835 195 NLISKPADFPEDN-SDGRRFYTDGPNVHEYLQQMHREVFE-GHDLINVGEMSSTSLEHCIRYSRP 257
                                                   ***********96.89***********************8.************************ PP

                                     TIGR02403 261 eekeLsmvftfhhLkvDyengekwtkakfdfaklkeilkewqeelqkengwnalflnnhDqPrav 325
                                                   e+keLsm+f+fhhLkvDy+n +kw++a+fdf +lk+il++wq+++q+++gwnalf++nhDqPr+v
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835 258 ESKELSMTFNFHHLKVDYPNLQKWVRADFDFLALKRILSDWQTGMQAGGGWNALFWCNHDQPRVV 322
                                                   ***************************************************************** PP

                                     TIGR02403 326 srfgddeeyreesakmlaaaihllrGtpyiyqGeeiGmtnpkfekiedykDveslnaykalkkkg 390
                                                   srfg+d+e+r+ sakml++a+h+l+Gtp++yqGee+Gmtnp f++i++y+Dve+ln+++ ++++g
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835 323 SRFGHDGEHRVVSAKMLGTALHFLQGTPFVYQGEELGMTNPGFDHIDQYRDVETLNIFRLKREAG 387
                                                   ***************************************************************** PP

                                     TIGR02403 391 kseeevlailkaksrDnsrtpmqWdeeknaGfstekpwlkvaknykeinvekeledeesvfkfyk 455
                                                    s+ + +a +++ksrDn+rtpm+W+ e naGfs+ +pw+ v +n+++inv ++l+d +sv+++y+
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835 388 SSDVDNMAAIMQKSRDNGRTPMHWNTEPNAGFSAVEPWIGVPANAAQINVAHQLDDPDSVLHHYR 452
                                                   ***************************************************************** PP

                                     TIGR02403 456 kliklrkeeeviaeGeyealekddpkvlaylrelkkekllvlanfsgeekkvklpeelks....a 516
                                                   +li+lr++e+++++G y++l+ ++ +++ay+re + e+llvl+nf+g+ ++v+lp e+ +    +
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835 453 QLIALRRSETLMSDGVYRQLLPEHTQIWAYVREGQGERLLVLNNFYGTPCEVELPPEVINesmvQ 517
                                                   *******************************************************9998878888 PP

                                     TIGR02403 517 kvllsnyeeaekk.aklelkPyeaivle 543
                                                   ++++sny +   + +++ l+Pye++vl+
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_835 518 SLVISNYPDCPPRnRQVFLRPYESFVLR 545
                                                   999******9988799********9975 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (544 nodes)
Target sequences:                          1  (549 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.03
# Mc/sec: 9.88
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources