Aligments for a candidate for catB in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Aligments for a candidate for catB in Pseudomonas fluorescens FW300-N1B4

Align muconate cycloisomerase (EC 5.5.1.1) (characterized)
to candidate Pf1N1B4_5805 Muconate cycloisomerase (EC 5.5.1.1)

Query= metacyc::MONOMER-14643
         (382 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5805
          Length = 429

 Score =  705 bits (1819), Expect = 0.0
 Identities = 361/386 (93%), Positives = 371/386 (96%), Gaps = 4/386 (1%)

Query: 1   MLATAIESIETIIVDLPTIRPHKLAMHTMQNQTLVIIRVRCADGIEGIGESTTIGGLAYG 60
           M ATAIESIETIIVDLPTIRPHKLAMHTMQNQTLVIIRVRCADGIEGIGESTTIGGLAYG
Sbjct: 44  MRATAIESIETIIVDLPTIRPHKLAMHTMQNQTLVIIRVRCADGIEGIGESTTIGGLAYG 103

Query: 61  NESPDSIKTNIDKHFAPLLIGQDSGNVNAAMLRLERSIRGNTFAKSGIETALLDAHGKRL 120
           NESPDSIKTNIDKHF PLLIGQDSGNVNAAMLRLERSIRGNTFAKSGIE+ALLDA GKRL
Sbjct: 104 NESPDSIKTNIDKHFTPLLIGQDSGNVNAAMLRLERSIRGNTFAKSGIESALLDAQGKRL 163

Query: 121 GLPVSELLGGRVRDALPVAWTLASGDTEKDIAEAEKMLDLRRHRIFKLKIGAGEVNRDLA 180
           GLPVSELLGGRVRDALPVAWTLASGDT KDIAEAE+MLDLRRHRIFKLKIGAGEVNRDLA
Sbjct: 164 GLPVSELLGGRVRDALPVAWTLASGDTAKDIAEAEQMLDLRRHRIFKLKIGAGEVNRDLA 223

Query: 181 HVIAIKKALGDRASVRVDVNQAWDEAVALRACRILGTNGIDLVEQPISRNNRGGMARLNA 240
           HVIAIKKALGDRASVRVDVNQAWDEAVALRACRILG+NGIDL+EQPISRNNR GM RLN 
Sbjct: 224 HVIAIKKALGDRASVRVDVNQAWDEAVALRACRILGSNGIDLIEQPISRNNRAGMVRLNT 283

Query: 241 MSPAPIMADESIECVEDAFNLAREGAASVFALKIAKNGGPRAVLRTASIAEAAGIALYGG 300
           +SPAPIMADESIECVEDAFNLAREGAASVFALKIAKNGGPRAVLRTA+IAEAAGI LYGG
Sbjct: 284 VSPAPIMADESIECVEDAFNLAREGAASVFALKIAKNGGPRAVLRTAAIAEAAGIGLYGG 343

Query: 301 TMLEGGLGTMASAHAFVTLNKLAWDTELFGPLLLTEDILSEPLVYRDFELHVPNTPGLGL 360
           TMLEGG+GT+ASAHAFVTLN L WDTELFGPLLLTEDILSEPLVYRDF+LHVP TPGLGL
Sbjct: 344 TMLEGGIGTLASAHAFVTLNTLGWDTELFGPLLLTEDILSEPLVYRDFQLHVPQTPGLGL 403

Query: 361 SLDEERLAFFRRDK----TSTAIHQA 382
           SLDEERLAFFRRDK    TSTA+HQA
Sbjct: 404 SLDEERLAFFRRDKVSRTTSTAVHQA 429


Lambda     K      H
   0.319    0.136    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 563
Number of extensions: 13
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 382
Length of database: 429
Length adjustment: 31
Effective length of query: 351
Effective length of database: 398
Effective search space:   139698
Effective search space used:   139698
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources