Alignments for a candidate for gtsA in Pseudomonas fluorescens FW300-N1B4

GapMind for catabolism of small carbon sources

Alignments for a candidate for gtsA in Pseudomonas fluorescens FW300-N1B4

Align ABC transporter for D-Glucose-6-Phosphate, periplasmic substrate-binding component (characterized)
to candidate Pf1N1B4_596 Glucose ABC transport system, periplasmic sugar-binding protein

Query= reanno::WCS417:GFF4324
         (428 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_596
          Length = 435

 Score =  699 bits (1805), Expect = 0.0
 Identities = 348/435 (80%), Positives = 380/435 (87%), Gaps = 7/435 (1%)

Query: 1   MNAINRLAVAISIASL-----FPLSAFAADSKGTVEVVHWWTSGGEKAAVDVLKAQVEKD 55
           MNAI+RLA  IS+ASL     FP+SA AAD+KG+VEVVHWWTSGGEKAAVDVLKAQVEKD
Sbjct: 1   MNAISRLATVISLASLLPVAAFPVSALAADAKGSVEVVHWWTSGGEKAAVDVLKAQVEKD 60

Query: 56  GFVWKDGAVAGGGGATAMTVLKSRAVAGNPPGVAQIKGPDIQEWASTGLLDTDVLKDVAK 115
           GF WKDGAVAGGGG+TAMTVLKSRAVAGNPPGVAQIKGPDIQEW STGLL TD LK+V+K
Sbjct: 61  GFTWKDGAVAGGGGSTAMTVLKSRAVAGNPPGVAQIKGPDIQEWGSTGLLSTDTLKEVSK 120

Query: 116 EEKWDSLLDKKVSDTVKYEGDYVAVPVNIHRVNWLWINPEVFKKAGITKNPTTLQEFYAA 175
            E WD+LL KKVSDTVKYEGDYVAVPVNIHRVNWLWINPEVFKKAGI K PTTL+EFYAA
Sbjct: 121 SEGWDNLLIKKVSDTVKYEGDYVAVPVNIHRVNWLWINPEVFKKAGIDKAPTTLEEFYAA 180

Query: 176 GDKLKAAGFIPLAHGGQPWQDSTVFEAVVLSVMGADGYKKALVDLDNGALTGPEMVKALT 235
           GDKLKAAGFI LAHGGQPWQDSTVFE VVLSVMGADGYKKALVDLD   L+GPEM KA T
Sbjct: 181 GDKLKAAGFIALAHGGQPWQDSTVFEDVVLSVMGADGYKKALVDLDQKTLSGPEMAKAFT 240

Query: 236 ELKKVATYMDVDGKGQDWNLEAGKVINGKAGMQIMGDWAKSEWTAAKKVAGKDYECVAFP 295
           ELKKV  YMD +  G+DWN+ A  VINGKAGMQ+MGDWAKSEWTAAKKVAGKDY+CV FP
Sbjct: 241 ELKKVTGYMDPNRAGRDWNIAAADVINGKAGMQMMGDWAKSEWTAAKKVAGKDYQCVPFP 300

Query: 296 GTDKAFTYNIDSLAVFKQK--DKGTAAGQQDIAKVVLGENFQKVFSINKGSIPVRNDMLN 353
           GT+KAFTYNIDS+AVFK K   KG  A QQD+AKV LG++FQKVFSINKGSIPVR DMLN
Sbjct: 301 GTEKAFTYNIDSMAVFKLKADRKGDIAAQQDLAKVALGKDFQKVFSINKGSIPVRTDMLN 360

Query: 354 KMDSYGFDSCAQTAAKDFLADAKTGGLQPSMAHNMATTLAVQGAFFDVVTNYINDPKADP 413
            M   GFD+CAQ +AKDFLAD KTGGLQPSMAHNMAT+LAVQGA FDVVTN++ND  ADP
Sbjct: 361 DMSGLGFDACAQASAKDFLADEKTGGLQPSMAHNMATSLAVQGAIFDVVTNFMNDKNADP 420

Query: 414 ADTAKKLGAAIKSAK 428
           A  + +L +A+K+A+
Sbjct: 421 AKASAQLASAVKAAQ 435


Lambda     K      H
   0.314    0.131    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 723
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 428
Length of database: 435
Length adjustment: 32
Effective length of query: 396
Effective length of database: 403
Effective search space:   159588
Effective search space used:   159588
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources