Protein AO353_04460 in Pseudomonas fluorescens FW300-N2E3
Annotation: AO353_04460 PTS N-acetyl-D-glucosamine transporter
Length: 838 amino acids
Source: pseudo3_N2E3 in FitnessBrowser
Candidate for 16 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| N-acetyl-D-glucosamine catabolism | nagF | hi | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized) | 100% | 100% | 1629.8 | D-trehalose PTS system, I, HPr, and IIA components | 46% | 669.5 |
| D-glucosamine (chitosamine) catabolism | nagF | hi | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized) | 100% | 100% | 1629.8 | D-trehalose PTS system, I, HPr, and IIA components | 46% | 669.5 |
| trehalose catabolism | treEIIA | med | D-trehalose PTS system, I, HPr, and IIA components (characterized) | 46% | 98% | 669.5 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| D-fructose catabolism | fruI | med | Fructose PTS system (E-I, HPr, and E-IIA components) (characterized) | 40% | 78% | 434.9 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| sucrose catabolism | fruI | med | Fructose PTS system (E-I, HPr, and E-IIA components) (characterized) | 40% | 78% | 434.9 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| D-cellobiose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 40% | 93% | 119.8 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| D-glucose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 40% | 93% | 119.8 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| lactose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 40% | 93% | 119.8 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| D-maltose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 40% | 93% | 119.8 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| sucrose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 40% | 93% | 119.8 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| trehalose catabolism | crr | lo | glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized) | 40% | 93% | 119.8 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| N-acetyl-D-glucosamine catabolism | nagEIIA | lo | Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) | 37% | 88% | 106.7 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| D-glucosamine (chitosamine) catabolism | nagEIIA | lo | Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) | 37% | 88% | 106.7 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| D-maltose catabolism | malEIIA | lo | Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale) | 37% | 88% | 106.7 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| N-acetyl-D-glucosamine catabolism | crr | lo | Putative PTS system sugar phosphotransferase component IIA (characterized, see rationale) | 38% | 97% | 90.9 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
| D-glucosamine (chitosamine) catabolism | crr | lo | Putative PTS system sugar phosphotransferase component IIA (characterized, see rationale) | 38% | 97% | 90.9 | N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) | 100% | 1629.8 |
Sequence Analysis Tools
View AO353_04460 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MHNNNKDLTLSAPLSGPVLTLAKVPDPVFASGAMGDGIAIDPLNNTLHAPCAGVVVHVAR
TGHAVTLRADNGAELLLHLGLDTVELQGEGFSMLVKEGTRVSNGQALLRFDLDQVAQGCK
SLVSLLVLTNSEDFQVLPITLKSVKVGEPLLHIVPRTTHSAQVEADSSGAEVHGHIRIIH
RGGLHARPAALIRQTAHLFNSKSQLHFAGKSASCDSLIGLMGLGIGEQDEVQVSCKGADA
KAALQALLNALSTAVNDDSHAAAPTPIAQRTRTAEAGVLNGVCAAPGLVGGPLFQLAAIP
LPEDTGKHNAEEQLQALDRALEQVRSEIRETLSHAKKHKHTEEEQIFAAHLALLEDPALL
EAAIQSIDQGSAATHAWSQSIEAQCEVLQQLGNPLLAERANDLRDLRQRVLRALLGQDWH
YDVPAGAIVAAHELTPSDLLQLSQQGVAGLCMAEGGATSHVAILARGKGLPCLVALSASL
LQQPQGQSVVLDADGGRLELTPDSQRLEQVAQAQREHLQRRERQQAQAHTPAHTRDGLRI
EVAANVASSNEAADALKGGADGVGLLRTEFLFVDRQTAPDEQEQRQAYQAVLDAMGDKSV
IIRTIDVGGDKQLDYLPLPAEANPVLGLRGIRMAQVRPELLDQQLRALLQVSPLQRCRIL
LPMVTEVDELLYIRQRLDALCAELALTQRLELGVMIEVPAAALLAEQLAEHADFLSIGTN
DLSQYTLAMDRDHAGLAARVDALHPALLRLIAQTCIGAAKHQRWVGVCGALASDPLATPV
LIGLGISELSVSPPQVGEIKERVRQLDAADCRRFSATLLNLSSATAVRHACHQHWPLS
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory