GapMind for catabolism of small carbon sources

 

Protein AO353_05300 in Pseudomonas fluorescens FW300-N2E3

Annotation: FitnessBrowser__pseudo3_N2E3:AO353_05300

Length: 481 amino acids

Source: pseudo3_N2E3 in FitnessBrowser

Candidate for 21 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arabinose catabolism xacF hi 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 95% 100% 910.6
D-galacturonate catabolism dopDH hi 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 95% 100% 910.6
D-glucuronate catabolism dopDH hi 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 95% 100% 910.6
D-xylose catabolism dopDH hi 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (characterized) 95% 100% 910.6
L-fucose catabolism aldA med lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) 41% 99% 362.5 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-rhamnose catabolism aldA med lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) 41% 99% 362.5 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-threonine catabolism aldA med lactaldehyde dehydrogenase (EC 1.2.1.22); 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized) 41% 99% 362.5 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-isoleucine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 36% 95% 281.6 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
myo-inositol catabolism mmsA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 36% 95% 281.6 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
propionate catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 36% 95% 281.6 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-threonine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 36% 95% 281.6 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-valine catabolism iolA lo malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized) 36% 95% 281.6 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-valine catabolism mmsA lo methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized) 34% 99% 279.3 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-arginine catabolism putA lo 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; L-glutamate gamma-semialdehyde dehydrogenase; EC 1.2.1.88 (characterized) 35% 90% 273.1 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-arginine catabolism rocA lo 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; L-glutamate gamma-semialdehyde dehydrogenase; EC 1.2.1.88 (characterized) 35% 90% 273.1 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-citrulline catabolism putA lo 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; L-glutamate gamma-semialdehyde dehydrogenase; EC 1.2.1.88 (characterized) 35% 90% 273.1 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-citrulline catabolism rocA lo 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; L-glutamate gamma-semialdehyde dehydrogenase; EC 1.2.1.88 (characterized) 35% 90% 273.1 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-proline catabolism putA lo 1-pyrroline-5-carboxylate dehydrogenase 2; P5C dehydrogenase 2; L-glutamate gamma-semialdehyde dehydrogenase; EC 1.2.1.88 (characterized) 35% 90% 273.1 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-arginine catabolism astD lo N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized) 36% 94% 231.1 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-citrulline catabolism astD lo N-succinylglutamate 5-semialdehyde dehydrogenase; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase; SGSD (uncharacterized) 36% 94% 231.1 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6
L-lysine catabolism amaB lo Putative aldehyde dehydrogenase transmembrane protein; EC 1.2.1.3 (characterized, see rationale) 35% 91% 228.4 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) 95% 910.6

Sequence Analysis Tools

View AO353_05300 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

VADVKRFDNFIGGQWVAGAEYSININPSELSDVIGEYAKADLAQVNAAIDAARAAFPAWS
TSGIQARSDALDKVGSEILARREELGTLLAREEGKTLPEAIGEVSRAGNIFKFFAGECLR
LSGDYLPSVRPGVNVEVTREALGVVGLITPWNFPIAIPAWKIAPALAYGNCVVLKPAELV
PGCAWALAEIISRAGFPAGVFNLVMGSGRVIGDAMVNSPKVDGISFTGSVGVGRQIAVNC
VSRQAKVQLEMGGKNPQIILDDADLKQAVELSVQSAFYSTGQRCTASSRLIVTAGIHDKF
VEAMAERMKSIKVGHALKSGTDIGPVVSQAQLEQDLKYIDIGQSEGARLVSGGGLVTCDT
EGYYLAPTLFADSEAAMRISREEIFGPVANIVRVADYDAALAMANDTEFGLSAGIATTSL
KYANHFKRHSQAGMVMVNLPTAGVDYHVPFGGRKGSSYGSREQGRYAQEFYTVVKTSYIG
S

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory