GapMind for catabolism of small carbon sources

 

Protein AO353_13360 in Pseudomonas fluorescens FW300-N2E3

Annotation: AO353_13360 ABC transporter ATP-binding protein

Length: 238 amino acids

Source: pseudo3_N2E3 in FitnessBrowser

Candidate for 21 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine catabolism braG hi ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 61% 97% 278.9 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-glutamate catabolism braG hi ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 61% 97% 278.9 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-histidine catabolism braG hi ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 61% 97% 278.9 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-isoleucine catabolism livF hi ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 61% 97% 278.9 Branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW 47% 206.8
L-leucine catabolism livF hi ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 61% 97% 278.9 Branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW 47% 206.8
L-valine catabolism livF hi ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) 61% 97% 278.9 Branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW 47% 206.8
D-alanine catabolism AZOBR_RS08250 hi Leucine//isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale) 61% 100% 275.4 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-proline catabolism AZOBR_RS08250 hi Leucine//isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale) 61% 100% 275.4 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-phenylalanine catabolism livF hi high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF (characterized) 60% 100% 265 ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM 53% 243.8
L-alanine catabolism braG med High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 59% 100% 257.7 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-serine catabolism braG med High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 59% 100% 257.7 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-threonine catabolism braG med High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 59% 100% 257.7 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-proline catabolism HSERO_RS00900 med ABC transporter ATP-binding protein (characterized, see rationale) 52% 96% 224.6 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-serine catabolism Ac3H11_1692 med ABC transporter ATP-binding protein (characterized, see rationale) 52% 96% 224.6 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-tyrosine catabolism Ac3H11_1692 med ABC transporter ATP-binding protein (characterized, see rationale) 52% 96% 224.6 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-histidine catabolism natE med NatE aka LivF aka SLR1881, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) 47% 95% 189.5 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-leucine catabolism natE med NatE aka LivF aka SLR1881, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) 47% 95% 189.5 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-proline catabolism natE med NatE aka LivF aka SLR1881, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) 47% 95% 189.5 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-isoleucine catabolism natE med NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 44% 96% 188.7 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-valine catabolism natE med NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 44% 96% 188.7 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0
L-phenylalanine catabolism livG lo High-affinity branched-chain amino acid transport ATP-binding protein LivG aka B3455, component of Leucine; leucine/isoleucine/valine porter (characterized) 32% 99% 124.4 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 60% 265.0

Sequence Analysis Tools

View AO353_13360 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MTQPILELKAIDVFYGPIQALKKVSLHINEGETVSLIGSNGAGKSTLLMSIFGQPRAAGG
QIVYRGVDITHKSSHYIASNGIAQSPEGRRVFPDMTVEENLLMGTIPIGDQYATEDMQRM
FELFPRLKERRNQRAMTMSGGEQQMLAIARALMSRPKLLLLDEPSLGLAPIVVKQIFATL
RELAGTGMTIFLVEQNANHALKLSDRAYVMVNGEIRLSGTGKELLVNEEVRNAYLGGH

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory