Protein AO353_27065 in Pseudomonas fluorescens FW300-N2E3
Annotation: FitnessBrowser__pseudo3_N2E3:AO353_27065
Length: 415 amino acids
Source: pseudo3_N2E3 in FitnessBrowser
Candidate for 10 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| ethanol catabolism | etoh-dh-c | med | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) (characterized) | 37% | 90% | 265.8 | vanillin dehydrogenase, cytochrome c component | 37% | 262.7 |
| ethanol catabolism | adhBqn | lo | Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 (characterized) | 37% | 91% | 260 | vanillin dehydrogenase, cytochrome c component | 37% | 262.7 |
| D-cellobiose catabolism | gadh2 | lo | Gluconate 2-dehydrogenase cytochrome c (characterized, see rationale) | 37% | 98% | 248.8 | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) | 37% | 265.8 |
| D-gluconate catabolism | gadh2 | lo | Gluconate 2-dehydrogenase cytochrome c (characterized, see rationale) | 37% | 98% | 248.8 | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) | 37% | 265.8 |
| D-glucose catabolism | gadh2 | lo | Gluconate 2-dehydrogenase cytochrome c (characterized, see rationale) | 37% | 98% | 248.8 | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) | 37% | 265.8 |
| lactose catabolism | gadh2 | lo | Gluconate 2-dehydrogenase cytochrome c (characterized, see rationale) | 37% | 98% | 248.8 | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) | 37% | 265.8 |
| D-maltose catabolism | gadh2 | lo | Gluconate 2-dehydrogenase cytochrome c (characterized, see rationale) | 37% | 98% | 248.8 | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) | 37% | 265.8 |
| sucrose catabolism | gadh2 | lo | Gluconate 2-dehydrogenase cytochrome c (characterized, see rationale) | 37% | 98% | 248.8 | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) | 37% | 265.8 |
| trehalose catabolism | gadh2 | lo | Gluconate 2-dehydrogenase cytochrome c (characterized, see rationale) | 37% | 98% | 248.8 | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) | 37% | 265.8 |
| 2-deoxy-D-ribose catabolism | drdehyd-cytc | lo | cytochrome c component of deoxyribose dehydrogenase (characterized) | 34% | 89% | 239.2 | alcohol dehydrogenase (cytochrome c) (EC 1.1.2.8) | 37% | 265.8 |
Sequence Analysis Tools
View AO353_27065 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MKRLLSSLGTAVGLAVSLMAIQQAQAADQQQIKRGEYLARAADCMACHTAPGGAPYAGGL
PIVSPFGTIYGTNITPDKDHGIGQYSDDEFFAALTEGKRRDGANLYPAMPYTSYHLMPRE
DSDAIHAYLQTVAPINRAAPVTRLSFPFNVRLGLMGWNMLYGKDVKLTPAEGKSEDWKRG
QYLVDVLGHCGECHTPRGLPGAMQQDKRLTGGLLNGYLAPSLLANDLAARGWTHQDLSSF
LKHGMSAQGTMFNEMFPVFHNSTQNLNEPDLASMATFLLGDPPPQARVLSEVPLDKMTES
ARRGRQDYLNVCAGCHGVNGEGKPHIAVAMQGNTTLRLEDPRNLLRVIEDGIGEQKFAGF
ERMQPMPGFADKLSNQQMTDLISYLRQAWGGQPADLLIGQIEQLKADAPVEHKAH
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory