GapMind for catabolism of small carbon sources

 

Aligments for a candidate for pimF in Pseudomonas fluorescens FW300-N2E3

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate AO353_00510 AO353_00510 multifunctional fatty acid oxidation complex subunit alpha

Query= metacyc::MONOMER-20678
         (699 letters)



>lcl|FitnessBrowser__pseudo3_N2E3:AO353_00510 AO353_00510
           multifunctional fatty acid oxidation complex subunit
           alpha
          Length = 715

 Score =  345 bits (886), Expect = 3e-99
 Identities = 227/692 (32%), Positives = 352/692 (50%), Gaps = 33/692 (4%)

Query: 23  VNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADITEFGKPPQPP------- 75
           VN  +      +   V+A  AD +V+ +++      FI GADITEF    + P       
Sbjct: 29  VNKFNRLTLNELRSAVDAIKADASVKGVIVSSGKDVFIVGADITEFVDNFKLPDAELVAG 88

Query: 76  --ALNDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEAKLGLPEVKLGLLPG 133
               N + +  E+   PT+AAI+G ALGGGLE+ L   +RV    AK+GLPEVKLG+ PG
Sbjct: 89  NLEANKIFSDFEDLNVPTVAAINGIALGGGLEMCLAADYRVMSTTAKIGLPEVKLGIYPG 148

Query: 134 AGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVV--ENLVAGAVAFAKKVLA- 190
            GGT RLPR +G + A++ I  G      +ALK G V+ VV  E L   A+   K+ ++ 
Sbjct: 149 FGGTVRLPRIIGADNAIEWIAAGKENRPEDALKVGAVDAVVAPEQLQEAALELLKRAISG 208

Query: 191 --EKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTKKARGLEAPFACADAIGAAIDLPFE 248
             + +  R+ + +  KL A +   +  T       +      AP      I  A +   +
Sbjct: 209 EFDYKAKRQPKLEKLKLNAIEQMMAFETAKGFVAGQAGPNYPAPVEAIKTIQKAANFGRD 268

Query: 249 EGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPRPVSRVAIIGAGTMGG 308
           + L+ E  GF+KL  +  +++    F  ++E  K     D    + V + A++GAG MGG
Sbjct: 269 KALEVEANGFVKLAKTSAAQSLIGLFLNDQELKKKAKAYDEIA-KDVKQAAVLGAGIMGG 327

Query: 309 GIAMSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLPPDAPAKRMALITGLVG 368
           GIA   A+ G P+ + +  E  +++GL    K       +G +     A+ +  I   + 
Sbjct: 328 GIAYQSASKGTPILMKDINEHGIEQGLAEAAKLLVGRVDKGRMTAAKMAEVLNGIRPTLS 387

Query: 369 LENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSYLSIDEIAATTKRPQDV 428
             +    DL++EAV E   VK+ V   V+   K G +LASNTS +SI  +A   KRP++ 
Sbjct: 388 YGDFGHVDLVVEAVVENPNVKQAVLAEVEDKVKEGTILASNTSTISISLLAKALKRPENF 447

Query: 429 LGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVVVGVCDGFVGNRMLAAR 488
           +GMHFF+P ++M L E++RG K++  A+ T V+ AKK+ K P+VV  C GF+ NR+L   
Sbjct: 448 VGMHFFNPVHMMPLVEVIRGEKSSEQAIATTVAYAKKMGKNPIVVNDCPGFLVNRVLFPY 507

Query: 489 SKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWRSR---------KDRGI 539
                KL+  G    ++D V+ KFG PMGP  + D+ G+D G   R         + +  
Sbjct: 508 FGGFAKLVSAGVDFVRIDKVMEKFGWPMGPAYLMDVVGIDTGHHGRDVMAEGFPDRMKDD 567

Query: 540 KSEIADALCEAGRFGQKTGKGYYKYE---QGSRAPMPDPEVETLINDTLAKLGLKRRDIT 596
           +    D L EA R GQK GKG+Y YE   +G +  + DP V     + L  +  ++R++T
Sbjct: 568 RRSAVDVLYEAKRLGQKNGKGFYAYETDKRGKQKKVADPSV----LEVLKPIVFEQREVT 623

Query: 597 DEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGPMHYADSVGLKHI 656
           DE+I+  M+ P+  E  R LE+ I    ++ D+  +YG G+P +RGG + Y D++G+   
Sbjct: 624 DEDIINWMMIPLCLETVRCLEDGIVETAAEADMGLVYGIGFPPFRGGALRYIDAIGVAEF 683

Query: 657 AERLSAYAKATNDPSLEPAPLLARLAAEGKTF 688
                 YA         P   L  +A  G++F
Sbjct: 684 VALADQYADL--GALYHPTAKLREMAKNGQSF 713


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1016
Number of extensions: 65
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 715
Length adjustment: 39
Effective length of query: 660
Effective length of database: 676
Effective search space:   446160
Effective search space used:   446160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory