Aligments for a candidate for Pf6N2E2_5403 in Pseudomonas fluorescens FW300-N2E3

GapMind for catabolism of small carbon sources

Aligments for a candidate for Pf6N2E2_5403 in Pseudomonas fluorescens FW300-N2E3

Align ABC transporter for D-Alanine, permease component 2 (characterized)
to candidate AO353_04610 AO353_04610 amino acid ABC transporter permease

Query= reanno::pseudo6_N2E2:Pf6N2E2_5403
         (375 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_04610
          Length = 393

 Score =  684 bits (1765), Expect = 0.0
 Identities = 340/375 (90%), Positives = 363/375 (96%)

Query: 1   VRAWVFQVVTVVAVIALGWFLFDNTQTNLQHRGITSGFGFLERSAGFGIAQHLIDYTEAD 60
           VRAW+FQ++T+VAVI++GW+LF+NTQTNLQHRGITSGF FLERSAGFGIAQHLIDYTE+D
Sbjct: 19  VRAWLFQIITIVAVISMGWYLFNNTQTNLQHRGITSGFSFLERSAGFGIAQHLIDYTESD 78

Query: 61  SYARVFLIGLLNTLLVTFIGVILATILGFIIGVARLSQNWIISKLATVYVEVFRNIPPLL 120
           SYARVF+IGLLNTLLVTFIGVILATILGFI+GVARLS+NWII+KLATVYVE FRNIPPLL
Sbjct: 79  SYARVFVIGLLNTLLVTFIGVILATILGFIVGVARLSKNWIINKLATVYVETFRNIPPLL 138

Query: 121 QILFWYFAVFLSMPGPRAAHNFGDTFFVSSRGLNMPAALVAEGFWPFVISVVLAIVAIVL 180
           QILFWYFAVFL+MPGPR +HNF DTFFVSSRGLNMPAA +A GFW FVIS+V+AIVAIVL
Sbjct: 139 QILFWYFAVFLTMPGPRNSHNFADTFFVSSRGLNMPAAQMANGFWAFVISIVVAIVAIVL 198

Query: 181 MTRWANKRFEATGEPFHKFWVGLALFLVIPALSALLFGAPVHWEMPELKGFNFVGGWVLI 240
           M RWANKRFEATG PFHKFWVGLALFL+IPAL AL+FG P+HWEMP+L+GFNFVGGWVLI
Sbjct: 199 MCRWANKRFEATGVPFHKFWVGLALFLLIPALCALIFGVPLHWEMPKLQGFNFVGGWVLI 258

Query: 241 PELLALTLALTVYTAAFIAEIVRSGIKSVSHGQTEAARSLGLRNGPTLRKVIIPQALRVI 300
           PELLALTLALTVYTAAFIAEIVRSGI SVSHGQTEAARSLGLRNGPTLRKVIIPQALRVI
Sbjct: 259 PELLALTLALTVYTAAFIAEIVRSGINSVSHGQTEAARSLGLRNGPTLRKVIIPQALRVI 318

Query: 301 IPPLTSQYLNLAKNSSLAAGIGYPEMVSLFAGTVLNQTGQAIEVIAITMSVYLAISISIS 360
           IPPLTSQYLNLAKNSSLAAGIGYPEMVSLFAGTVLNQTGQAIEVIAITMSVYLAISISIS
Sbjct: 319 IPPLTSQYLNLAKNSSLAAGIGYPEMVSLFAGTVLNQTGQAIEVIAITMSVYLAISISIS 378

Query: 361 LLMNWYNKRIALIER 375
           LLMNWYNKRIALIER
Sbjct: 379 LLMNWYNKRIALIER 393


Lambda     K      H
   0.328    0.141    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 646
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 393
Length adjustment: 30
Effective length of query: 345
Effective length of database: 363
Effective search space:   125235
Effective search space used:   125235
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources